中文摘要：

目的 探讨细粒棘球绦虫（Echinococcus granulosus，Eg）亲肌肉抗原重组蛋白诱导ICR小鼠产生的免疫应答及其对Eg原头节攻击感染的保护性作用。方法 36只雄性ICR小鼠随机分为A（重组蛋白免疫组）、B（空质粒蛋白免疫组）和C（PBS对照组）等3组，每组12只。3组小鼠分别经皮下注射重组蛋白（10 μg/只）、空质粒蛋白（10 μg/只）和PBS（100 μl/只），第2和4周同法加强免疫2次。末次免疫后2周，每只小鼠腹腔接种细粒棘球蚴原头节50个进行攻击感染。感染后20周，剖杀小鼠, 分离并称重细粒棘球蚴组织，计算囊重减少率。取脾脏，分离脾细胞，流式细胞仪检测脾脏CD4＋ T和CD8＋ T淋巴细胞亚群百分比。脾细胞体外经脾细胞悬液、Eg粗抗原 （EgAg）和伴刀豆球蛋白A （ConA）刺激培养4～5 h后，四甲基偶氮唑盐比色法（MTT法）检测T淋巴细胞增殖情况。 结果 感染后20周，A组小鼠包囊质量［（0.019±0.036） g］明显低于C组［（0.058±0.057） g］（P〈0.05），囊重减少率为69.1%；CD4＋ T和CD8＋ T细胞亚群百分比分别为（29.7±0.9）%和（9.7±0.8）%，均高于C组［（11.6±1.4）%和（7.8±0.2）%］（P〈0.01和P〈0.05），CD4＋/CD8＋比值（3.061±0.015）也显著高于C组（1.487±0.106）（P〈0.01）。未经刺激时，A组小鼠脾T淋巴细胞增殖水平（0.237±0.009）高于C组（0.159±0.005）（P〈0.01）；用EgAg和ConA刺激后，A组小鼠脾T淋巴细胞增殖水平［（0.283±0.008）和（0.325±0.025）］均高于C组［（0.203±0.002）和（0.244±0.006）］（P〈0.01）。 结论 棘球蚴亲肌肉抗原重组蛋白可诱导免疫小鼠脾脏T淋巴细胞增殖，CD4＋ T细胞亚群在重组蛋白诱导的小鼠抗Eg原头节攻击感染的保护性免疫机制中起一定作用。

英文摘要：

Objective To investigate the immune response and the protection in mice induced by the recombi-nant myophilin protein of Echinococcus granulosus. Methods Thirty-six male ICR mice of 6-8 weeks old were randomly divided into groups A, B and C each with 12. The mice in the 3 groups were subcutaneously immunized with Eg myophilin protein, blank plasmid protein or PBS, respectively, by 3 times and challenged with protoscoleces of E. granulo-sus 2wk after the last vaccination. Mice were sacrificed 20wk after the infection, the hydatid cysts were collected for mea-suring the weight reduction. Spleens were obtained and the splenocytes were separated and cultured in vitro with EgAg or ConA stimulus for 4-5 h. The subsets of CD4＋ and CD8＋ T cells were measured by FACsort. The proliferation of spleno-cytes was determined by MTT method with blank plasmid and PBS as control. Results The average weight of the hy-datid cysts in the immunized group decreased by 69.1% in comparison to the blank plasmid and PBS groups. The CD4＋ subset [（29.7±0.9）%] and CD8＋ subset [（9.7±0.8）%] in group A increased significantly than group C, [（11.6±1.4）%] and [（7.8±0.2）%] respectively （P〈0.01 or 〈0.05）. The ratio of CD4＋/CD8＋ subsets in group A （3.061±0.015） was also higher than group C （1.487±0.106） （P〈0.01）. Without stimulation, the proliferation of T lymphocytes in group A（0.237±0.009）was high-er than group C （0.159±0.005） （P〈0.01）, with EgAg or ConA stimulus, it was also higher in group A than that of group C（P〈0.01）. Conclusion The recombinant myophilin protein of E. granulosus can induce the proliferation of splenocytes and Th1 response in mice, and the CD4＋ T cells subset may bear a part in the induced protection against the challenge of protoscoleces.