中文摘要：

目的：探索一种在体测定大鼠心肌缺血再灌注损伤后心肌梗死面积的方法,并与传统的离体染色方法进行了比较,确定其可行性。方法：结扎雄性成年Sprague-Dawley大鼠冠状动脉前降支,给予缺血30 min,再灌注6 h,用超声心动图评价大鼠的心功能,用生化分析仪测定血清中的肌酸激酶（creatine kinase,CK）活性。再灌注24 h后,用离体和在体两种方法进行TTC（triphenyltetrazolium chloride,2,3,5-氯化三苯基四氮唑）-Evans blue双染,比较两种方法的成功率、心梗面积/缺血区面积（in-farct area/risk area,IA/RA）与血清CK活性的相关性。结果：在体染色法的IA/RA与离体染色法没有显著性差异,但成功率高。其IA/RA与血清CK活性的相关性高于离体染色法。结论：在体染色法与离体染色法相比,操作简易,能更准确地反映心肌缺血再灌注损伤的程度。

英文摘要：

Objective：To develop a new method of measuring infarct size by staining hearts in vivo after ischemia-reperfusion,and compare with the traditional method of staining in vitro to determine its feasibility.Methods：Adult male Sprague-Dawley rats were subjected to 30 min myocardial ischemia and 6 h reperfusion by ligation of the left anterior descending coronary artery.Cardiac function was evaluated by echocardiography and serum creatine kinase（CK） activity was also measured by a biochemical analyzer.After reperfusion for 24 h,hearts were staining by triphenyltetrazolium chloride（TTC）-Evans blue in vitro or in vivo.Infarct area（IA） / risk area（RA） was calculated.Finally we compared the success rate of the two methods,and made correlation analysis of the IA / RA with serum CK activity.Results：There was no significant difference in IA / RA staining by the two different methods.However,success rate of staining in vivo was significant higher than that of staining in vitro.Besides,compared with staining in vitro,there was a stronger correlation between IA / RA and CK activity by staining in vivo.Conclusion：The method of staining in vivo is much easier to operate,and it can evaluate the damage by myocardial ischemia-reperfusion injury more accurately than that of staining in vitro.