中文摘要：

瞄准：为了描绘差别，从胆汁的闭锁(BA ) 在肝表示了基因侧面病人包括，查明在 BA 的致病起一个中央作用的基因，功能的范畴和小径，并且为 BA 识别新奇预示的标记。方法：从控制病人，新生的 cholestasis 病人，和 BA 病人在岁时的肝织物样品 < 60 d， 60-90 d，并且 > 90 d 为 DNA microarray 分析被分享。生物信息学分析被执行用，基因本体论的系列测试簇，和小径查找者软件。反向抄写的聚合酶链反应被执行在选择基因证实变化。在 RRAS 基因表示和 40 个 BA 病人的预后之间的关系在 2 年的后续研究被分析。结果：4 识别重要基因表示侧面能充满信心地把 BA 肝织物与正常和另外的 diseased 肝纸巾分开。包括的基因主要涉及发炎反应和细胞的矩阵的重建。与 BA 联系的重要小径首先涉及自体免疫的反应， T 淋巴细胞的激活和它的相关 cytokines。RRAS， POMC， SLC26A6 和 STX3 基因是在 BA 的致病的重要规章的模块。RRAS 的表示否定地与黄疸的消除率被相关并且断然与胆管炎的出现率相关。结论：自体免疫的反应由淋巴细胞可以玩的 T 调停了在 BA 的致病的一个重要角色。RRAS 基因是在 BA 的致病的一个重要规章的模块，它可以为 BA 用作一个新奇预示的标记。

英文摘要：

AIM：To characterize the differentially expressed gene profiles in livers from biliary atresia （BA） patients including,ascertain genes,functional categories and pathways that play a central role in the pathogenesis of BA,and identify the novel prognostic markers for BA.METHODS：Liver tissue samples from control patients,neonatal cholestasis patients,and BA patients at the age of 60 d,60-90 d,and 90 d were pooled for DNA microarray analysis.Bioinformatics analysis was performed using,series test cluster of gene ontology,and Pathway-Finder software.Reverse-transcription polymerase chain reaction was performed to confirm changes in selected genes.Relation between RRAS gene expression and prognosis of 40 BA patients was analyzed in a 2-year follow-up study.RESULTS：The 4 identified significant gene expression profiles could confidently separate BA liver tissue from normal and other diseased liver tissues.The included genes were mainly involved in inflammation response and reconstruction of cellular matrix.The significant pathways associated with BA were primarily involved in autoimmune response,activation of T lymphocytes and its related cytokines.The RRAS,POMC,SLC26A6 and STX3 genes were important regulatory modules in pathogenesis of BA.The expression of RRAS was negatively correlated with the elimination rate of jaundice and positively correlated with the occurrence rate of cholangitis.CONCLUSION：Autoimmune response mediated by T lymphocytes may play a vital role in the pathogenesis of BA.The RRAS gene is an important regulatory module in the pathogenesis of BA,which may serve as a novel prognostic marker for BA.