中文摘要：

人的颈的癌症 HeLa 房间有功能的线粒体。最近的研究建议了那 mitochondrial 新陈代谢在肿瘤房间增长起一个必要作用。不过，房间怎么协调 mitochondrial 动力学和房间周期前进，尚待被澄清。为了调查在函数和房间周期规定， mitochondrial 基因表达式侧面和细胞的 ATP 层次是的 mitochondrial 之间的关系，在现在的学习由房间周期进步分析决定了。HeLa 房间被浆液饥饿在 G0/G1 阶段同步，并且由恢复浆液文化重入房间周期，时间功课实验被执行分析 mitochondrial 抄写管理者的表示， mitochondrial 基因， mitochondrial 膜潜力(MMP ) ，细胞的 ATP 层次，和房间骑车前进。结果证明当逮捕 G0/G1 房间在包含浆液的媒介被刺激时， DNA 和表示的数量在开始在 2 h 时间点增加的线粒体 mRNA 和蛋白质铺平，而 MMP 和 ATP 水平在 4 点提高了 h。而且，在浆液触发了房间周期以后， cyclin D1 表示开始在 4 h 增加。ATP 合成 inhibitoroligomycintreatment 压制了 cyclin D1 和 cyclin B1 表示层次并且堵住了房间周期前进。一起拿，我们的结果建议增加的 mitochondrial 基因表示层次，氧化 phosphorylation 激活，和细胞的 ATP 满足增加是为被触发的重要事件房间周期。最后，我们证明 mitochondrial 基因表示层次和细胞的 ATP 内容紧在调整房间增长被调整并且可能玩一个中央角色。

英文摘要：

Human cervical cancer HeLa cells have functional mitochondria. Recent studies have suggested that mitochondrial metabolism plays an essential role in tumor cell proliferation. Nevertheless, how cells coordinate mitochondrial dy- namics and cell cycle progression remains to be clarified. To investigate the relationship between mitochondrial func- tion and cell cycle regulation, the mitochondrial gene ex- pression profde and cellular ATP levels were determined by cell cycle progress analysis in the present study. HeLa cells were synchronized in the G0/G1 phase by serum star- vation, and re-entered cell cycle by restoring serum culture, time course experiment was performed to analyze the expression of mitochondrial transcription regulators and mitochondrial genes, mitochondrial membrane potential （MMP）, cellular ATP levels, and cell cycle progression. The results showed that when arrested G0/G1 cells were stimu- lated in serum-containing medium, the amount of DNA and the expression levels of both mRNA and proteins in mitochondria started to increase at 2 h time point, whereas the MMP and ATP level elevated at 4 h. Furthermore, the cyelin D1 expression began to increase at 4 h after serum triggered cell cycle. ATP synthesis inhibitor-oligomycin-- treatment suppressed the cyclin D1 and cyclin B1 expres- sion levels and blocked cell cycle progression. Taken to- gether, our results suggested that increased mitochondrial gene expression levels, oxidative phosphorylation activa- tion, and cellular ATP content increase are important events for triggering cell cycle. Finally, we demonstrated that mitochondrial gene expression levels and cellular ATP content are tightly regulated and might play a central role in regulating cell proliferation.