中文摘要：

建立了一种以SAX固相小柱萃取和高效液相色谱（ HPLC）法同时检测土壤中咪唑烟酸、甲基咪草烟、咪草酸甲酯和咪唑乙烟酸4种咪唑啉酮类除草剂残留的方法。考察了不同提取剂、pH、固相萃取（ SPE）小柱和淋洗液体积等因素对回收率的影响。结果表明：采用SAX 固相萃取小柱，以V（乙腈）∶V（水）＝5∶3混合溶液为提取剂，6 mL甲醇为淋洗液时，在pH＝3条件下，样品的提取及净化效果较好。淋出液浓缩后用甲醇定容，过滤膜后经HPLC检测。添加回收试验结果表明：在0？02～0．5 mg/kg添加水平下，4种除草剂的平均回收率在86％～109％之间，相对标准偏差（RSD）≤3．3％（n ＝5）。咪唑烟酸、甲基咪草烟和咪唑乙烟酸在土壤中的定量限（LOQ）均为0．01 mg/kg，咪草酸甲酯的LOQ为0．02 mg/kg。

英文摘要：

A new method for determining residues of imazapyr, imazapic, imazamethabenz-methyl and imazethapyr in soil was developed by solid-phase extraction （ SPE ） cleanup and high performance liquid chromatography（ HPLC） detection. Various extraction reagents, solvents at different pH values, various type of SPE and the elution volumes were compared. Extraction with V （ acetonitrile ）∶V（water） =5∶3 at pH 3, with SAX cartridge SPE purification of the extracts were found to be the most suitable conditions. Determination was done with HPLC coupled with diode array detector （ DAD ） . Mean recoveries of four imidazolinone herbicides at fortification levels of 0 . 02-0 . 5 mg/kg were between 86% -109% （RSD≤3. 3%, n=5）. Under the optimal conditions, limits of quantification in＆amp;nbsp;soil were 0？01 mg/kg for imazapyr, imazapic and imazethapyr, and 0. 02 mg/kg for imazamethabenz-methyl （ S/N=3 ） .