中文摘要：

采用超滤—离子层析法精制猪硫酸软骨素,离子对反相高效液相色谱法测定其含量,红外吸收光谱法测定其结构。研究了超滤液温度、超滤压力、超滤液pH对猪硫酸软骨素精制时膜通量和得率的影响。结果表明：超滤—离子层析技术可应用于精制猪硫酸软骨素。猪硫酸软骨素超滤的工艺条件：以微孔滤膜处理原料液,超滤膜的截留分子量10 kD,超滤压力0.25 MPa,超滤温度25℃,溶液pH为9。产物纯度为95.74%,产率为80.15%。该方法精确度和准确度都较高,可用于猪硫酸软骨素的精制。

英文摘要：

Porcine chondroitin sulfate was refined by UF membrane-ion-exchange-chromatography.Its structure was determined with infrared spectroscopy and its content was determined by Ion-pair RP—HPLC.The effects of ultrafiltrate temperature,ultrafiltration pressure and ultrafiltrate pH on the flux and yield of porcine chondroitin sulfate were studied when it was refined.UF membrane-ion-exchange chromatography can be used to refine porcine chondroitin sulfate.The process conditions of the refinement of porcine chondroitin sulfate： using microporous membrane to process raw material liquid,the molecular weight cutoff of UF membrane is 10 kD,ultrafiltrate pressure 0.25 MPa,ultrafiltrate temperature 25 ℃ and pH 9.Purity of products is 95.74% and yield 80.15%.This method is of high accuracy and can be used to refine porcine chondroitin sulfate.