中文摘要：

在碱性介质中，发现血红蛋白对luminol-hydrazine弱化学发光体系有显著的增强作用。结合流动注射法提出了luminol-hydrazine化学发光（FI-CL）测定血红蛋白的新方法，优化了化学发光检测条件和FI-CL系统的运行参数，考察了实际样品中可能含有的共存物质对血红蛋白测定的影响。结果表明，血红蛋白的线性范围为5．0×10^-9～6．0×10^-5g·mL^-1，检出限是5．8×10^-10g·mL^-1（9．0×10^-12mol·L^-1），分别对5．0×10^7和3．0×10^-6g·mL^-1的血红蛋白标准溶液平行八次测定所得RSDs分别为1．6％和1．5％。将提出的方法成功用于人血液和血清中血红蛋白含量的测定，加标回收率在83．0％～101．0％范围。结合化学发光光谱和紫外可见吸收光谱对血红蛋白催化luminol-hydrazine化学发光反应的机理进行了探讨。提出的方法具有灵敏度高、无需标记、操作简便、快速、易实现自动化操作等优点。

英文摘要：

A sensitive method to detect hemoglobin （Hb） by flow injection （FI） coupled with chemilumines- cence （CL） detection was described, which based on Hb strong enhancing effect on weak luminol-hydrazine CL system in alkaline medium. Parameters affecting the CL detection conditions and FI-CL system were opti- mized. The effects of possible coexisting substances in human blood and serum of detection Hb were evalua- ted. Under the optimum conditions, the net CL intensity versus Hb concentration was linear in the range of 5.0 × 10^-9 6.0 × 10^-5 g. mL-1 with the detection limit of 5.8× 10^-10 g . mL-1 （9.0 × 10^-12mol . L-1 ）. The relative standard deviations （RSDs） for 8 replicate determinations of 5.0 ×10^-7 and 3.0 × 10^-6 g . mL-1 Hb were 1.6% and 1.5%, respectively. In addition, the recoveries of Hb in human blood and serum were car- ried out and varied from 83.0% to 101.0%. The proposed method has been successfully applied for the deter- mination of Hb in healthy human blood and serum. The possible mechanism of Hb enhancing the weak CL emission of luminol-hydrazine system in NaOH solution was discussed by fluorescence spectrophotometer and UV-Vis spectrophotometer.