中文摘要：

基于牛血清白蛋白（BSA）在碱性介质中对luminol-H2O2体系化学发光的增敏作用，结合流动注射化学发光技术提出了一种快速、 灵敏检测BSA的新方法。在优化条件下，检测BSA的线性范围为2.5×10^-9～1.0×10^-6 mol·L^-1，检出限为5.8×10^-10 mol·L^-1，样品检测速率达112个·h×-1。对1.0×10^-7 mol·L^-1 BSA溶液重复测定11次，相对标准偏差RSD为0.8%。该方法已成功应用于实际样品牛血清中BSA含量的测定。结合化学发光光谱和紫外可见吸收光谱，对luminol-H2O2-BSA体系可能的反应机理进行了探讨。

英文摘要：

A new, rapid and sensitive method for the determination of bovine serum albumin （BSA） by flow-injection coupled with chemiluminescence detection was establish, which based on BSA enhancing effect on weak chemiluminescence system of Luminol-H202 in alkaline solution. Under the optimized conditions, linearity for BSA was found in the range of 2. 5 × 10^- 9 -1.0× 10^-6 mol · L^-1 , with the detection limit of 5.8× 10^-10 mol · L^-1. And the sampling detecting rate was about 112 samples ·h^-1. The relative standard deviation（ RSD） was found to be 0. 8% for 11 replicate determinations of 1.0× 10 7mol · L^-1.BSA. The proposed method has been satisfactorily applied to the determination of BSA in the bovine serum samples. The possible mechanism of Luminol-H2O2-BSA was studied by chemiLuminescence spectrum and UV-visible spectrum.