中文摘要：

目的：探讨HIV/AIDS肺脾气虚证患者的血液代谢谱特征。方法：采用高效液相色谱质谱联用技术结合偏最小二乘法-判别分析（PLS-DA）,t检验分析技术,比较24例HIV/AIDS肺脾气虚证患者和20例健康志愿者血液代谢产物差异。结果：两组在血液代谢轮廓上得到了良好的区分,通过查询人类代谢组库,推测出8种可能生物标志物。结论：高效液相色谱质谱联用结合PLS-DA模式识别的代谢组学方法可以将HIV/AIDS肺脾气虚证与健康人血液代谢轮廓上进行良好区分,在指导中医证型的诊断方面有良好的应用价值。

英文摘要：

Objective ： Todiscuss the metabolism of blood spectrum characteristics in HIV/AIDS Lung spleen - deficiency syndrome. Methods ： Use high performance liquid chromatography mass spectrometry technology - combined with partial least squares discriminant analysis （PLS- DA）, t- test analysis technology to comparethe blood generation product differences of 24 cases of HIV/AIDS Lung spleen - deficiency syndrome patients and 20 cases of healthy volunteers. Results ： The patients and healthy volunteers can be separated in the score plots of OPLS - DA models. Through comparing the human metabolome database, weinferred eight possible biomarkers. Conclusion： High performance liquid chromatography mass spectrometry combined with PLS - DA metabolomics methods of pattern can well distinguish HIV/AIDS Lung spleen - deficiency syndrome and healthy volunteers on blood metabolic profile, and has a good application value in guiding syndrome diagnosis of traditional Chinese medicine.