中文摘要：

目的评价一种可以快速定量检测疟原虫及虫种鉴别的荧光定量PCR法。方法将SYBRGreenI法与TaqMan探针法检测疟原虫进行比对，评价定量的准确性及方法的敏感度。以PCR-DNA测序法为金标准，评价SYBRGreenI法分型的准确性。结果两个方法的定量拷贝检测数无显著性差异（P〉0．05）。SYBRGreenI法检测疟原虫的最低检测下限为1×10^2copy／mL至5×10^2copy／mL之间。与PCR-DNA测序法比较，SYBRGreenI法能准确的对感染的疟原虫虫种做准确的鉴定。结论SYBRGreenI染料法定量可同时进行人体疟原虫的定量检测和虫种鉴别，且成本低廉，操作简便快速，可以在疟疾的高发地区推广使用。

英文摘要：

Objective To evaluate a SYBR Green I real-time PCR method for quantitative detection and species identification of human Plasmoth＇um spp. Methods The SYBR Green I method was compared with TaqMan real-time PCR for investigation of the accuracy and sensitivity. At the same time, it was compared with the PCR-DNA sequencing for accuracy of the species identification. Results There were no significant difference between quantitative results of the SYBR Green I method and the TaqMan real-time PCR （P〉0.05）. The limit range of detection located from 1 x 102 copy/mL to 5 x 102 copy/mL. Compared with the PCR-DNA sequencing, it was accurate to identify the species of Plasmodium spp. Conclusion The SYBR Green I real- time PCR method was lower cost, convenient and rapid for human Plasmodium spp. quantitative detection and species identification in the high prevalence area.