中文摘要：

制备多柔比星白蛋白纳米粒（DOX／BSANP），观察其药物缓释规律，探讨其对肝癌细胞的杀伤作用。去溶剂．化学交联法制备DOX／BSANP载药纳米粒；透射电镜观察其形态及粒径；分光光度法测定包封率及载药率；体外药物释放实验考察缓释特性；MTY法及FCM观察其对肝癌细胞的体外杀伤作用，动物实验观察其体内抑瘤效应。透射电镜下DOX／BSANP呈圆球形，分布均匀，直径约为120nm，动态光衍射测定其水合粒径约为170nm，包封率为82％，载药量为5％，体外药物释放实验显示，载药纳米粒中约50％的药物持续缓慢释放，可持续长达7d；MTT实验显示DOX／BSANP能显著抑制细胞的增殖，FCM检测显示其能诱导肿瘤细胞凋亡；体内抑瘤实验显示，载药纳米粒的疗效优于DOX原药的疗效。成功制备多柔比星白蛋白纳米粒。体内外实验证明DOX／BSANP纳米粒可有效抑制肝癌细胞Bel-7404的生长。

英文摘要：

To prepare DOX/BSANP, and to observe its antitumor effect on liver cancer cells in vitro and in vivo, DOX/BSANP drug loading nano-particle was prepared by desolvation-chemical crosslinking method. The characters of DOX/BSANPs such as morphology, drug loading efficiency, drug entrapment efficiency and drug release rate in vitro were investigated. The chemotherapy effects of the DOX/BSANPs on liver cells in vitro were evaluated by M3~I＂ assay and flow cytometry. The liver tumor xenograft model was estab- lished on nude mice to evaluate the antitumor effect in vivo. Tumor volumes and histological analysis were performed after treating by different methods. Under transmission electron microscope, DOX/BSANP looked spherical, and uniformly distributed, diameter was about 120 nm, hydrated particle size was about 170 nm determined by dynamic light diffraction, envelopment rate was 82% and drug loading rate was 5%. In vitro drug release experiment showed that about 50% drug in drug loading nano-particle could be released continuously and slowly for 7 days. MTY test showed that DOX/BSANP could significantly inhibit cell proliferation. FCM test showed that it could induce apoptosis of tumor cells. In vivo experiments showed that therapeutic effect of drug loading nano-particle was superior to that of nude DOX. DOX/BSANP was successfully prepared. Both in vitro and in vivo experiments showed that DOX/ BSANP could effectively inhibit the growth of liver cancer cell Bel-7404.