中文摘要：

目的：研究补益营卫方、转化生长因子β1（TGFp1）及碱性成纤维细胞生长因子（bFGF）对衰老人皮肤成纤维细胞c—Jun基因表达的影响。方法：通过连续传代培养复制衰老人皮肤成纤维细胞模型，通过中和抗体阻断TGFp1及bFGF后，利用荧光定量PCR法检测衰老人皮肤成纤维细胞C-Jun基因表达。结果：人皮肤成纤维细胞衰老时，C-Jun基因表达水平24h和48h比人年轻皮肤成纤维细胞分别下降了70％和71％，72h则两者的表达水平相当。衰老人皮肤成纤维细胞补益营卫药物处理后显著提高了c-Jun基因的表达，相对于衰老人皮肤成纤维细胞，其24h、48h分别提高了4．67倍和2．12倍，而至72h其表达水平相当。中和TGF-p1蛋白后，衰老人皮肤成纤维细胞c-Jun基因的表达水平24h和72h分别是年轻人皮肤成纤维细胞的2．4倍和1．7倍。而48h时，年轻人皮肤成纤维细胞的基因表达水平是衰老人皮肤成纤维细胞的9．2倍。衰老人皮肤成纤维细胞补益营卫药物处理后显著提高了c-Jun基因的表达，48h和72h时c—Jun基因的表达水平分别是衰老人皮肤成纤维细胞的2．3倍和1．5倍。中和TGF—p1和bFGF蛋白后，c—Jun基因的表达水平随着时间的不同被明显下调；与年轻人皮肤成纤维细胞相比，24h、48h和72h衰老人皮肤成纤维细胞c-Jun基因的表达水平分别下降了64．5％、94．2％和73．7％。衰老人皮肤成纤维细胞补益营卫药物处理后c-Jun基因的表达随着时间的不同相对于衰老人皮肤成纤维细胞被显著上调。24h、48h和72h分别是衰老人皮肤成纤维细胞的9．7倍、3．6倍和9．8倍。纵向比较来看，TGF-β1蛋白对衰老人皮肤成纤维细胞C-Jun基因的表达存在复杂的调节作用。bFGF蛋白对衰老人皮肤成纤维细胞c-Jun基因表达有明显的促进作用。结论：衰老时人皮肤成纤维细胞c-Jun基因的表达紊乱。补益营卫方对衰老人?

英文摘要：

Objective To study the c-Jun gene expression influenced by the recipe of supplementing Ying-wei and transforming growth factor-pl（TGF-β1） and basic fibroblast growth factor（bFGF） in the aging human skin fibroblasts. Methods Firstly,to copy the aging model of human skin fibroblasts by continuous passage culturing.Secondly,to detect the c-Jun gene expression of the aging human skin fibroblasts through the fluorescent quantitative PCR method,after neutralizing antibody blocking TGF-β1 and bFGF. Results When the human skin fibroblasts aging,the expressive level of c-Jun gene,compared to that of young skin fibroblasts,falls by 70% and 71% in 24h and 48h.But the expressive levels in 72h are both similar.As aging human skin fibroblasts treated by the recipe of supplementing Ying-wei,the c-Jun gene expression which has increased to 4.67 times and 2.12 times in 24h and 48h,has significantly improved,compared to the aging human skin fibroblasts.And the expressive levels of them are equivalent in 72h.After neutralizing TGF-β1 protein,c-Jun gene expressive level of the aging human skin fibroblasts is 2.4 times and 1.7 times as large as that of young skin fibroblasts in 24h and 72h.In 48h,the gene expressive level of young skin fibroblasts is 9.2 times as large as that of aging human skin fibroblasts.After using the recipe of supplementing Ying- wei,the c-Jun gene expression of aging human skin fibroblasts has improved obviously.The c-Jun gene expressive level of human skin fibroblasts raise by 2.3 times and 1.5 times in 48h and 72h.After neutralizing TGF-151 protein and bFGF protein,c-Jun gene expressive level is decreased obviously with different time;Compared with the young skin fibroblasts,c-Jun gene expression of the aging human skin fibroblasts falls by 64.5% ,94.2% and 73.7% in 24h,48h and 72h.As aging human skin fibroblasts treated by the recipe of supplementing Ying-wei,c-Jun gene expression has significantly increased over time.c-Jun gene expression in 24h,48h and 72h is of 9.7 times,3.6 times and 9.8 tim