中文摘要：

利用免疫细胞荧光、RNA干涉（RNA interfere,RNAi）、qRT-PCR、Western blot及细胞计数等方法,设计合成三对小分子干扰RNA（small interfering RNA,siRNA）（siRNA-hnRNP K-1,2,3）,对小鼠精原细胞中内源性的不均一性核糖核蛋白K（Heterogeneous ribonucleoproteins K,hnRNP K）进行敲低并对其沉默效率进行筛选,研究其对小鼠精原细胞存活的影响.结果表明,siRNA-hnRNP K-3对小鼠精原细胞具有80%以上的沉默效率,转染后发现细胞数目明显减少,说明沉默hnRNP K基因的表达对小鼠精原细胞的存活有显著影响.

英文摘要：

Through the immune cell fluorescence,RNAi（RNA interfere）,qRT-PCR,Western blot and cell counting methods,three pairs of siRNA（small interfering RNA）（siRNA-hnRNP K-1,2,3）were designed and synthesed to screen the silence efficiency that the endogenous hnRNP K of spermatogonium in mice were knockdown,and then studied the effect on spermatogonium survival.Results showed that the siRNA-hnRNP K-3had silence efficiency more than 80%,and the cell number decreased significantly from further transfection to spermatogonium in mice.The results showed that the hnRNP K gene knockdown had a significant effect on the survival of spermatogonium in mice.