中文摘要：

目的：初步探讨βB2晶状体蛋白（Crybb2）参与调节生殖过程的作用机制。方法：选取11-13厨龄βB2基因敲除（KO组，n=19）与野生型C57BL／C（WT组，n=23）雌性小鼠，阴道涂片观察动情周期变化；HE染色观察卵巢病理变化：光学显微镜下计数卵巢最大切面原始卵泡、初级卵泡、闭锁卵泡；Western blotting和免疫组织化学确定βB2晶状体蛋白在卵巢组织中的表达与定位。结果：βB2晶状体蛋白主要表达在Ⅵ组小鼠卵巢颗粒细胞内。与WT组小鼠相比，KO组小鼠卵巢相对重量减轻，动情周期紊乱，原始卵泡、初级卵泡减少，闭锁卵泡增多。结论：βＢ2基因敲除小鼠的动情周期及卵巢发育异常，βB2晶状体蛋白对小鼠卵巢的发育有重要影响。

英文摘要：

Objective： To explore the effects and mechanism of βB2-crystallin （Crybb2） in mouse ovary development and estrous cycle. Methods： Adult （11-13 weeks） βB2 gene knockout （KO group, n=19） and wild-type （WT group, n=23） female C57BL/C mice were used. Estrous cycle was determined through vaginal smear. HE staining was used to examine the effects of βB2 crystallin on the ovary tissue. The number of primordial, primary and atresic follicles was counted by using paraffin sections under optical microscope in the largest ovarian cross-section. Expression of βB2 crystallin in mouse ovary was assayed by Western blotting and immunohistochemistry. Results： The ovary weight inβB2 gene knockout mice reduced and demonstrated an irregular estrous cycle compared with WT group. The follicular atresia was increased, while the number of primordial and primary follicles was reduced in βB2 gene knockout mouse ovary. Expression ofβB2crystallin was mainly expressed in granulosa cells of WT mice, not in βB2 gene knockout mice. Conclusion： βB2 gene knockout mice displayed ovarian dysplasia, suggesting that βB2crystallin may play an important role in regulation of ovary development of mice.