通过观察烟草品种N.glauca、N.langsdorffii和杂种Mglauca×N.1angsdorffii的形态差异,利用IAA、6-BA、ABA等不同激素处理杂种mglauca×N.langsdorffii探究激素对于遗传瘤产生的影响。实验结果显示生长素能显著抑制实生幼苗的遗传瘤产生,而分裂素则明显促进遗传瘤的产生。本实验还首次从烟草N.glauca中诱导产生遗传瘤,这说明种间杂交并不是诱导产生遗传瘤的唯一因素。电镜扫描结果显示遗传瘤中的细胞分化很不规则,可能是由于细胞分化及细胞间协作失控引起的。
The morphology of N. effect of phytohormone on hybrid glauca, N. langsdorffii and N. glauca genetic tumor formation was analyzed. x N. langsdo~fii was detected, and the The result showed that exogenous cyto- kinin (N6-benzyladenine, BA) enhanced the tumor formation on hybrid seedling, while auxin (indole-3-acetic acid, IAA) inhibited. It was the first report on genetic tumor formation from N. glauca, suggesting that genetic factors may control tumor formation independent of the specific interspecific cross. The SEM assay showed that the tumor surface cells arranged irregularly and we proposed that the irregular arrangment of tumor cells was due to the loss of normal interaction and coordination between cells.