中文摘要：

沃尔巴克氏体是一种广泛分布于节肢动物及线虫体内的胞内共生菌,能通过多种方式参与宿主生殖调控。多位点序列分型技术（MLST）是一种基于核酸序列测定的细菌分型方法,该方法通过PCR技术扩增多个管家基因内部片段并测定其序列,进而分析菌株变异。该文对沃尔巴克氏体分型及系统发育分析研究现状以及重点对MLST方法在沃尔巴克氏体中的研究进展进行综述,并对MLST技术在蚊媒生物防治中的应用进行展望。

英文摘要：

Wolbachia is a genus of endosymbiotic alphaproteo bacteria infecting a wide range of arthropods and filarial nematodes, which can manipulates the reproductive mode of its hosts in various kinds of ways. Multilocus sequence typing （MLST） was proposed as a nucleotide sequence-based approach that could be applied to many bacteria. It analyzed allelic variation at multiple housekeeping loci by nucleotide sequencing of internal fragments. Here we make an overview on the state of Wolbachia genotypes and phylogeny, and the development of MLST analysis on Wolbachia. We also make a prospect on the application of MLST in the biological control of mosquito-borne diseases.