中文摘要：

随着蛋白质组学的快速发展,蛋白质的翻译后修饰引起了广泛的关注.其中蛋白质的糖基化修饰是最常见、最重要的蛋白质翻译后修饰之一,在许多生理和病理过程中起重要作用.同时获得蛋白糖基化氨基酸位点和糖链结构信息,对了解糖基化的生物学意义重大.

英文摘要：

Glycoslyation is one of the most important post-translational modifications.Although many glycosylation sites are detected through mass spectrometry,there is still lack of high-throughput bioinformatical tools for identification of intact glycopeptides.A novel software that is able to fully utilize the information derived from experiments of mass spectrometry was developed.The results reveal thousands non-redundant intact glycopeptides in a single 2D-LC run.