位置:成果数据库 > 期刊 > 期刊详情页
亚洲百合遗传转化受体系统的建立
  • 期刊名称:干旱地区农业研究.25(1):219-224,2007年1月
  • 时间:0
  • 分类:S682.29[农业科学—观赏园艺;农业科学—园艺学]
  • 作者机构:[1]旱区作物逆境生物学国家重点实验室/西北农林科技大学林学院,陕西杨凌712100
  • 相关基金:国家自然科学基金项目“百合ACC合成酶反义基因构建及其遗传转化”(30371013)
  • 相关项目:百合ACC合成酶反义基因构建及其遗传转化
中文摘要:

为了优化亚洲百合生根培养基配方,为亚洲百合转基因植株试管苗的生根移栽奠定一定的基础,以亚洲百合'普利安娜'(Lilium Asiatic hybrid cv.'Pollyanna')和'普瑞头'(Lilium Asiatic hybrid cv.'Prato')试管苗为材料,探讨5种不同激素的浓度组合对试管苗生根率、生根数量、最长根长的影响。结果表明:不同植物激素的组合和浓度对亚洲百合的生根有显著影响,且2个品种生根状况有一定的差异。在生根培养基为MS+2,4-D1.0mg/L+KT0.1mg/L+6-BA1.0mg/L+蔗糖30g/L+琼脂7g/L时,亚洲百合'普利安娜'平均生根率达到98.73%,生根数量达到6.53条,最长根长为4cm;生根培养基为:MS+NAA1.0mg/L+TDZ0.1mg/L+蔗糖30g/L+琼脂7g/L时,亚洲百合'普瑞头'平均生根率达到92.73%,生根数量达到6.63条,最长根长为3.77cm。最后得出:亚洲百合'普利安娜'最适宜的生根培养基为:MS+2,4-D1.0mg/L+KT0.1mg/L+6-BA1.0mg/L+蔗糖30g/L+琼脂7g/L;亚洲百合'普瑞头'的适宜生根培养基为:MS+NAA1.0mg/L+TDZ0.1mg/L+蔗糖30g/L+琼脂7g/L。

英文摘要:

In order to optimize the formulation of Asian lily rooting medium and lay a foundation for rooting and transplanting of Asian lily transgenic plants,Lilium Asiatic hybrid cv.' Pollyanna ' and Lilium Asiatic hybrid cv.' Prato ' test-tube seedlings were used to study the effect of combinations of five different hormone in different concentrations on rooting rate,root number,longest root length.The results showed that:a combination of different types and concentrations of plant hormones significantly affected Asiatic lily rooting,and the rooting status of the two species had some differences.When using the rooting medium:MS+2,4-D 1.0 mg/L+KT 0.1 mg/L+6-BA 1.0 mg/L+sucrose 30 g/L+ agar 7 g/L,the average rooting rate of Lilium Asiatic hybrid cv.' Pollyanna ' could reach 98.73%,the root number of them could reach 6.53 cm and the longest root length of them was 4 cm.When using the rooting medium:MS+NAA 1.0 mg/L+TDZ 0.1 mg/L+sucrose 30 g/L+ agar 7 g/L,the average rooting rate of Lilium Asiatic hybrid cv.' Prato ' could reach 92.73%,the root number of them could reach 6.63 cm and the longest root length of them was 3.77 cm.The most appropriate rooting medium of Lilium cv.' Pollyanna ' was:MS+2,4-D 1.0 mg/L+KT 0.1 mg/L+6-BA 1.0 mg/L+sucrose 30 g/L+ agar 7 g/L;the most suitable rooting medium for Lilium cv.' Prato ' was:MS+NAA 1.0 mg/L+TDZ 0.1 mg/L+ sucrose 30 g/L+agar 7 g/L.

同期刊论文项目
同项目期刊论文