中文摘要：

目的 观察大鼠海马胆囊收缩素（CCK）阳性（CCK＋）、阴性（CCK-）神经元电生理学特性的差异.方法 用CCK启动子-绿色荧光蛋白（GFP）的腺病毒标记CCK＋神经元与未被标记的CCK-神经元（分别为CCK＋组及CCK-组）,观察细胞形态,检测静息状态下的静息电位（Vm）、膜阻抗（Rm）、膜电容（Cm）、时间常数（Tau）；用100 pA（500 ms）去极化电流诱发CCK＋神经元和CCK-神经元的动作电位,记录动作电位的频率、时间间隔（ISI）、幅值（Amp）、动作电位达幅值中点的时间（HW）、动作电位上升（10％～90％）的时间（RT）、动作电位下降（90％ ～ 10％）的时间（DT）和发放频率；在灌流外液中加入50 μM AP-5和5μM NBQX,记录细胞自发抑制性突触后电流（sIPSC）的频率.结果 CCK＋组细胞长径、短径、胞体面积均大于CCK-组（P均＜0.05）.CCK＋组细胞静息状态下Rm大于CCK-组,Tau小于CCK-组,P均＜0.05.两组Vm、Cm比较无统计学差异.CCK＋组动作电位频率、HW大于CCK-组,ISI、RT小于CCK-组,P均＜0.05.两组Amp比较无统计学差异.CCK＋组神经元sIPSC的发生频率高于CCK-组,P＜0.05.结论 CCK＋神经元的传递速度快于CCK-神经元.

英文摘要：

Objective To observe the electrophysiological characteristics divergences of CCK positive and negative neurons on cultured rat hippocampus. Methods Using the CCK promoter-green fluorescent protein （GFP） of adenovirus labeled neurons as CCK ＋, and unlabeled as CCK -. The aim was to detect morphology, the resting potential （ Vm）, membrane resistance （Rm）, membrane capacitance （Cm）, time constant （Tan） of these two cells; and the neurons action potentials were evoked by 100 pA （500 ms） depolarizing current. Then recording the value： action potential frequency, interevent interval （ISI）, amplitude （Amp）, the action potential half-width was the time between two points at the midpoint of the peak amplitude from the AP threshold（ HW）, the action potential rise time （10 - 90%, RT）, the action potential decay time （90 - 10%, DT） and firing frequency. The perfusion extracellular fluid was filled with 50 μM AP-5 and 5 μM NBQX and then the spontaneous inhibitory postsynaptic current （sIPSC） was recorded. Results The long diameter in CCK ＋ cells were （22.85 ± 0.59） μm, short diameter （15.37 ± 0.30） μm, cell body area （347.61 ±12.12） μm2. The value in CCK - group were （20.62 ± 0.51 ） μm, （ 14.24 ± 0. 43 ） μm, （ 303.23 ± 14.30） μm2. The long diameter, short diameter and soma size of the two groups all had significant difference, P 〈 0. 05. The CCK ± group： Vm （ - 49.16 ± 1.94） mV, Cm （42.86±1.78） pF, Rm （601.5±35.71） MΩ, Tan （221.08 ±35.63） μs; the CCK-group were （ - 48.81 ±1.81） mV, （47.85±2.33） pF, （504.66±31.08） MΩ, （500.32±40.27） μs. The Rm and Tan of CCK ＋ neurons compared to CCK -cells, P 〈 0.05, but Vm and Cm had no obvious difference between the two group, P 〉0.05. The action potential frequency of CCK ± cells were （31.7 ± 1.89） Hz, ISI as （31.94 ± 0.57） ms, Amp （74.07 ± 3.16） mV, HW （2.60±0.38） ms, lit （7.44±0.84） ms, DT（3.51 ±0.73） ms; CCK- gr