中文摘要：

目的 研究细粒棘球绦虫原头节可溶性抗原（protoscolex soluble antigens,PSA）对树突状细胞（dendritic cells,DC）表型分化及分泌炎症因子的影响,探索寄生虫对宿主免疫逃避的机制。方法 运用免疫磁珠从健康BALB/c小鼠脾脏中分选DC,分别加入PSA（10μg/ml）、LPS（1μg/ml）、PSA（10μg/ml）＋LPS（1μg/ml）以及等体积PBS进行刺激24h,收集细胞及培养上清,利用流式细胞术检测DC表面活化分子的表达水平,并采用流式液相多重蛋白定量技术检测细胞因子分泌情况。结果 PSA组DC表面表达CD40,CD80,CD86,MHCⅡ的比例为（42.33±0.59）%、（71.08±1.61）%、（73.63±5.31）%、（70.20±1.01）%,较PBS组的（29.1±8.14）%、（48.81±1.69）%、（37.37±2.57）%、（26.83.08±3.55）%均明显升高（P〈0.05）,较LPS组的（61.33±4.73）%、（81.23±2.03）%、（85.40±1.57）%、（86.86±2.01）%均显著降低（P〈0.05）。PSA组培养上清中IL-6、TNF浓度为（53.67±6.23）pg/ml、（405.10±5.78）pg/ml,较PBS组的（9.44±4.06）pg/ml、（139.35±45.86）pg/ml、均显著升高（P〈0.05）,较LPS组的（48.23±7.85）pg/ml、（471.40±61.07）pg/ml均显著降低（P〈0.05）;且PSA＋LPS组IL-6、TNF浓度为（73.42±8.00）pg/ml和（508.54±20.68）,较PSA组、LPS组显著升高（P〈0.05）。结论 PSA能促进DC活化及炎症因子释放,从而诱导机体产生正向免疫应答。

英文摘要：

Objective Objectives To study the effects of soluble antigens （SAs） of the protoscolex of Echinococcus granulosus on the phertotypic differentiation of and inflammatory cytokine secretion by dendritic cells （DCs） and to examine the mechanism of immune regulation used by the parasite. Methods Immunomagnetic beads were used to sort DCs from the spleen of normal BALB/c mice and those DCs were then stimulated with SAs of the E. granulosus protoseolex （10μg/ml）, LPS （1μg/ml）, or SAs （10μg/ml） ＋ LPS （1 μg/ml） and an equal volume of PBS. The cells and superna tants were collected after 24 h. Flow cytometry was used to detect the expression of activation markers on the surface of DCs, and a cytometric bead array （CBA） was used to detect the levels of cytokines in the cultured supernatants. Results Of the DCs stimulated with SAs, 42.33±0.59% expressed CD40, 71.08±1.61% expressed CDS0, 73.63±5.31% ex pressed CD86, and 70. 20±1.01% expressed MHC II. These percentages were all significantly higher than those in DCs treated with PBS [29.1±8.14%, 48.81±1. 69%, 37.37±2.57%, and 26.83.08±3.55%] （P〈0.05） and significant ly lower than those in DCs treated with LPS [61.33±4.73%, 81.23±2.03%, 85.40±1.57%, and 86.86±2.01%] （P〈0.05）. After stimulation with SAs, the concentration of IL-6 in the cultured supernatants was 53.67±6.23 pg/ml and the concentration of and TNF was 405.10±5.78 pg/ml. These concentrations were significantly higher than those in DCs treated with PBS [9.44±4.06 pg/ml and 139.35±45.86 pg/ml] （P（0.05） and significantly lower than those in DCs treated with LPS [48.23±7.85 pg/ml and 471.40±61.07 pg/ml] （P（0.05）. In cells stimulated with SAs＋LPS, the level of IL-6 was 73.42±8.00 pg/ml and the level of TNF was 508.54±20.68 pg/ml. These levels were significantly higher than those in DCs stimulated with SAs and DCs treated with LPS （P〈0.05）. Conclusion Stimulation with SAs of the protoseolex of E. granulosus induced the activation of DCs