中文摘要：

目的研究Notch信号通路相关分子在颞下颌骨关节炎（temporomandibularjointarthritis，TMJOA）中的表达情况，探索该通路在TMJOA发生发展中的作用及机制。方法将72只昆明小鼠随机分为实验组、假手术组及正常组。实验组小鼠手术摘除右侧颞下颌关节盘，假手术组除不摘关节盘外其余操作同实验组，正常组不作处理。所有小鼠左侧颞下颌关节盘不作处理。术后1周、2周、4周每组处死8只小鼠，进行组织化学染色，根据骨关节炎的病理诊断标准评估TMJOA模型是否建立成功，选取成功的TMJOA软骨进行免疫组化染色检测Notch1（NICD1）、Jagged1、Hes1、Hes5的表达情况，并采用半定量方法评分。结果实验组髁突Notch1（NICD1）、Jagged1、Hes5术后表达量明显增加，且与TMJOA进展呈正相关；Hes1表达明显降低，第4周时有所增加。结论TMJOA模型中，Notch通路相关分子表达发生了明显变化，表明该通路在TMJOA发生发展中起着重要作用。

英文摘要：

Objective To investigate the expression of Notch signaling molecules in temporomandibular joint ar?thritis(TMJOA),and to explore the role and mechanism of Notch signaling pathway in the development and progressionof TMJOA.Methods72Kunming mice were randomly divided into experimental group,sham?surgery group and nor?mal group.In experimental group,discs on the right TMJ were subjected to total discectomy,sham?operation group un?derwent the same procedure without disc removal,while normal group serve as blank control.All the left temporoman?dibular joint discs were not treated.8mice in each group were sacrificed respectively at1week,2week,4week aftersurgery.Histological examinations were performed to assess success of TMJOA model,according to the pathologicalstandard of osteoarthritis diagnosis.Immunochemistry techniques were performed in the successful TMJOA cartilages toevaluate the expression levels of Notch1(NICD1),Jagged1,Hes1and Hes5.The scores were evaluated by semi?quantita?tive method.Results Notch1(NICD1),Jagged1and Hes5were activated in the experimental group with the expressionlevels increased dramatically over time.While Hes1expression was suppressed at the beginning of osteoarthritis butwas up?regulated afterwards.Conclusion Notch pathway?related molecular expression changed greatly in TMJOA mod?el,indicating that the pathway in the occurrence and development of TMJOA plays an important role.