中文摘要：

目的研究羧胺三唑对脂多糖诱导的RAW264．7细胞炎症模型中炎症因子的影响。方法脂多糖（1μg／ml）刺激生长良好的RAW264．7细胞，建立细胞炎症模型，并用不同浓度羧胺三唑处理。CCK-8法检测羧胺三唑对RAW264．7细胞的毒性作用，ELISA法检测细胞上清液中TNF-α、IL-1β和IL-6含量，分光光度法检测一氧化氮（NO）含量。结果羧胺三唑在2．5～40μmol／L的浓度范围内对RAW264．7细胞无毒性作用（P〉0．05）。脂多糖可以明显诱导RAW264．7细胞分泌TNF-α、IL-1β、IL-6和NO（P〈0．01），10、20、40μmol／L的羧胺三唑能够显著抑制脂多糖诱导的RAW264．7细胞释放TNF-α、IL-6和NO的水平（P〈0．05和P〈0．01），20、40μmol／L的羧胺三唑能够抑制脂多糖诱导的IL-1β的释放（P〈0．01）。结论羧胺三唑可以抑制脂多糖诱导的RAW264．7细胞炎症反应，其抗炎作用与减少炎症因子TNF—α,、IL-1β、IL-6和NO有关。

英文摘要：

Objective To investigate the effects of carboxyamidotriazole （CAI）on inflammatory factors in lipopolysaccharide（LPS）-induced RAW264.7 cells. Methods The inflammation model was established using LPS- induced RAW264. 7 cell, and different concentration of CAI was added in the culture medium. The cytotoxicity of CAI was measured by cell counting kit-8 （ CCK-8 ） method. The contents of TNF-α, IL-1β and IL-6 in the culture supernatant were determined by ELISA method, and nitric oxide （NO） content was assayed by spectrophotometric method. Results CAI showed no cytotoxicity on RAW264. 7 ceils at the concentrations from 2. 5 to 40 μmol/L（P 〉 0.05）. LPS induced markedly increased secretions of TNF-α, IL-1β, IL-6 and NO from RAW264.7 ceUs（P 〈 0.01 ）. CAI （10,20,40 μmoL/L）exhibited the inhibitory effects on TNF-α, IL-6 and NO releases in LPS-induced RAW264. 7 cells （P 〈 0. 05 and P 〈 0. 01 ）, and CAI （ 20,40 μmol/L） inhibited LPS-induced IL-1β release （ P 〈 0. 01 ）. Conclusion CAI has anti-inflammtory effects, which might be mediated by decreasing the inflammatory factors such as TNF-α, IL-1β, IL-6 and NO.