对山核桃中类脂褐素的提取方法进行了研究,摸索了山核桃中类脂褐素的特征荧光光谱,找到了山核桃中类脂褐素测定的最佳前处理方法,并以此为基础通过单因素试验和L9(3^4)正交试验,研究了提取方式、提取温度、提取时间以及提取溶剂pH对类脂褐素提取率的影响。结果表明采取乙醚进行除油前处理和氯仿:甲醇:磷酸缓冲溶液(2:1:1)的提取溶剂,提取时间为2.0h,提取温度50℃,提取液pH6.0时山核桃类脂褐素的提取率最高。
in the experiment,we studied the extraction method of lipofuscin - like pigments from Chinese walnuts (carya cathayensis), explored their feature fluorescence spectrum of lipofuscin - like pigments, and found out their suitable pretreatment of samples before extraction. And then on this basis the single factor test and orthogonal experiment L9 ( 3^4 ) design methods were applied to study the influence of the variables on the lipofuscin - like pigments such as extraction mode, temperature and time, as well as PH of extraction solvent. The results showed that, the highest extraction rate of lipofuscin - like pigments could be gained in the conditions ( extraction solvent with the proportion of chloroform : methanol : phosphate buffer (2:1 : 1 ), extraction time of 2.0 h, extraction temperature of 50℃ and extraction solution of pH 6.0) after oil removal pretreatment with ether.