中文摘要：

目的：探究不同剂量柚皮素（naringenin）对人下咽癌FADU细胞的作用及其相关机制。方法：以不同剂量（0、100、200、400、800μg/m L）柚皮素对FADU细胞进行处理,倒置显微镜观察细胞形态学变化;cck-8法检测药物作用后不同时间（24、48、72 h）FADU细胞增殖的变化;流式细胞仪检测柚皮素作用48 h细胞凋亡的变化;采用Transwell趋化小室体外侵袭实验测定48 h后细胞的侵袭能力的变化;蛋白免疫印迹法检测48 h后细胞内PI3K/AKT信号通路凋亡相关蛋白表达的影响。结果：cck-8法检测发现柚皮素抑制FADU细胞增殖作用明显,呈浓度和时间依赖性;流式细胞结果发现200,400,800μg/m L柚皮素作用48 h后,FADU细胞的凋亡率显著增高,差异有统计学意义（P〈0.05）。Transwell体外侵袭实验发现,随着柚皮素浓度增加,FADU细胞穿透聚碳酸酯滤膜的能力逐渐减弱,柚皮素有效的抑制FADU细胞在体外的侵袭能力,呈浓度依赖性。同时,蛋白免疫印迹法凋亡相关蛋白检测结果表明,FADU细胞内BCL2、P-AKT和P-PI3K蛋白表达均受到明显抑制。结论：柚皮素能有效抑制人下咽癌FADU细胞的增殖、体外侵袭能力,诱导细胞凋亡;其机制与下调PI3K/AKT信号通路凋亡相关蛋白表达有关。

英文摘要：

Objective： To explore the influence of different concentrations ofnaringenin on the growth ofhypopharyngeal carcinoma FADU cells and its action mechanisms. Methods： The role of naringenin on hypopharyngeal carcinoma on the growth of FADU cells was assessed after treated at the concentrations of 0, 100, 200, 400, 800 μg/ml for 24, 48 and 72 hours. Using the CCK8 method, the effects of naringenin on the growth of FADU cells was measured after treated in different dose. Using the flow cytometry, the effects of narin- genin on the apoptosis of FADU cells was measured after treated in different dose. Using the transwell method, the effects of naringenin on the migration ability of FADU ceils was measured after treated in different dose. Using the western-blot method, the expression of BCL2, P-AKT, PI3K was measured. Results： Compared with the control group, cell proliferation rate, migration ability, expression of BCL2, P-AKT, PI3K decreased with the increase of dose and cell apoptosis rate increases with the dose increased significantly. Conclusion： The naringenin has inhibitory effect on FADU cell growth, migration and induced apoptosis of cells. Its mechanism could be related to the reduction of protein expression in PI3K/AKT signal pathways.