中文摘要：

【目的】克隆、分析和原核表达编码棉铃虫Helicovcrpa armigera（Hü bner）信息素结合蛋白HarmPBP2的cDNA，并纯化得到HarmPBF2蛋白。【方法】以棉铃虫触角为材料，通过RT-PCR技术以获得编码中棉铃虫信息素结合蛋白HarmPBP2基因成熟蛋白开放阅读框序列，使用半定量RT-PCR对表达谱进行研究，并在使用pGEX-4T-1表达载体在BL21（DE3）系统中进行原核表达，使用GSTrap FF预装柱进行纯化并切去标签。【结果】克隆了命名为HarmPBP2（GenBank登录号：EU647241）的棉铃虫信息素结合蛋白基因，该序列全长457bp，编码149个氨基酸残基，前端是一段25个氨基酸长的信号肽，推测蛋白具有昆虫信息素结合蛋白典型的6个保守的半胱氨酸残基的特征。棉铃虫HarmPBP2在雌雄成虫的触角、足和翅中都有表达，另外在雌虫下颚须也有表达。雌雄组织表达量顺序相同，触角中表达量最高，翅中次之，足中最少。雌虫组织中表达量高于雄虫。构建的原核表达载体pGEX／HarmPBP2，在大肠杆菌BL21（DE3）中成功地表达出一个分子量约为40kD的融合蛋白，经亲和层析纯化与酶切得到去标签的HarmPBP2蛋白。【结论】克隆、分析和表达了棉铃虫信息素结合蛋白HarmPBP2的cDNA序列，并对其进行纯化，为进一步研究其分子结构和功能奠定了基础，同时对其表达谱进行了研究。

英文摘要：

[Objective] A gene named HarmPBP2 encoding pheromone binding protein 2 was cloned from Helicoverpa armigera and expressed in prokaryotic system, and purified HarmPBP2 was obtained. [Method] The HarmPBP2 gene was cloned in H.armigera using reverse transcription-polymerase chain reaction （RT-PCR）. And its spatio-temporal expression pattern was studied by semi-quantitative RT-PCR analysis. HarmPBP2 was expressed using pGEX-4T-1/BL21 （DE3） prokaryotic expression system, and purified using GSTrap FF. [Result] The full length ofHarmPBP2 （GenBank loucs： EU647241） was 457 bp, encoding 149 amino acids, including 25 aa of signal peptide. Sequencing and analysis indicated that HarmPBP2 was characterized by six conservative Cys, which shared typical feature of OBP with those of other insects. Further analysis showed that HarmPBP2 may belong to the family of PBP. HarmPBP2 was expressed in antenna, leg and wing in both male and female, and also expressed in female maxillary palpus. The expression quantity was antenna〉wing〉leg, and the female〉male. The molecular weight of recombinant protein of pGEX-4T-1/HarmPBP2 was about 40 kD. About 14 kD HarmPBP2 was obtained by affinity chromatography and cleavage of GST-tag. [Conclusion] HarmPBP2 was cloned and expressed it in prokaryotic expression system ,and then the expressed protein was purified, which is helpful for further researches on molecular structure and function of HarmPBP2, and the expression pattern was studied by Semi-quantitative RT-PCR analysis.