中文摘要：

目的对不同饲料原料中真菌污染状况进行研究。方法从全国不同地区的代表性饲料生产企业采集豆粕、棉籽粕、麦麸和玉米干酒糟及其可溶物（DDGS）4种饲料原料样品，采用平板稀释法接种，对影响真菌分离的关键因素进行筛选，并对各样品的真菌污染状况进行分析。结果含氯霉素0．1g／L的马铃薯葡萄糖琼脂、10-稀释度分别为从饲料中分离真菌的最适培养基和稀释度。饲料原料中真菌污染率范围为31％（DDGS）～88％（麦麸）；不同地区同种饲料原料、同一地区不同种类的饲料原料样品受真菌污染的程度各异。不同地区、不同种类饲料原料中重要产毒真菌黄曲霉和镰刀菌的污染水平也各不相同，东北地区黄曲霉和镰刀菌的污染水平最高，华南地区最低；麦麸和棉籽粕污染水平最高，DDGS最低；各种饲料中黄曲霉的平均污染水平（25％）高于镰刀菌（11％）。结论我国饲料原料样品受黄曲霉和镰刀菌的污染值得关注，需在饲料生产加工过程中，加强对真菌污染的控制。

英文摘要：

Objective To survey the fungi contamination of different feed ingredients in China. Methods Soybean meal, cottonseed meal, wheat bran and distillers dried grains with soluble （DDGS） were collected from feed enterprises in different part of the country. Feed ingredient samples were diluted and inoculated and the key factors were screened which influenced the isolation. Results Potato dextrose agar （containing ehloramphenicol 0. 1 g/L） and 10-1 dilution were the optimum culture medium and dilution respectively. The range of fungi contamination was from 31% （DDGS） to 88% （wheat bran）. The fungi contamination frequencies of feed ingredients varied from region to region, and varied from kind to kind. The contamination of Aspergillus flavus and Fusarium spp. were also different in the regions and sample types. The average contamination percentage of Aspergillus flavus （25%） is higher than Fusarium spp. （11% ） , and their contamination level is highest in northeast China and lowest in south China, wheat bran and cottonseed meal were the highest and DDGS was the lowest. Conclusion The fungi contamination, including Aspergillus flavus and Fusarium spp. of feed ingredients all over the country should be pay more attention. It is recommended that prevention of feed ingredients from fungi contamination is needed.