研究以0.2和20μmol/L Na2SeO3及一氧化氮供体硝普钠(SNP)处理对水稻根中根系活力和硫代巴比妥酸反应产物含量,愈创木酚过氧化酶(GPX)、超氧化物歧化酶(SOD)、过氧化氢酶(CAT)以及抗坏血酸过氧化物酶(APX)活性等生理生化指标的影响。结果表明,1μmol/L SNP处理显著提高0.2μmol/L Na2SeO3处理下水稻根系活力,SNP通过促进CAT酶活性,缓解了膜脂过氧化;在20μmol/L Na2SeO3处理下,1μmol/L SNP明显促进SOD酶活性,但显著降低APX酶活性,显著降低根系活力。NO对水稻根中Se引起的抗氧化活性变化具有调节作用,根系活力可以作为评价抗氧化活性的重要参考。
In this study, we reported some antagonist effects of exogenous nitric oxide on oxidative stress of rice induced by selenium. The root activity, the contents of TBARS and the activities of GPX, SOD, CAT and APX in roots of rice seedlings treated with varied concentrations of selenium and 1 μmol / L SNP were investigated. The results showed that the root activity increased by treatment of SNP in 0.2 μmol / L Na2 SeO3 group. SNP alleviated significantly the lipid peroxidation in rice seedlings via increasing CAT activities in rice root. In 20 μmol / L Na2 SeO3 treated rice seedlings, SNP aggravated signifi- cantly the root activity loss via promoting SOD activities and repressing APX activity. Taken together, results suggested that NO regulates antioxidative activity caused by selenium in roots of rice seedlings. Root activity can be used as reference index of evaluating antioxidative activity.