中文摘要：

目的探讨p38 MAPK对人主动脉平滑肌细胞Ⅰ型和Ⅲ型胶原表达的影响。方法分别使用药理学抑制剂和siRNA干预细胞。药理学抑制剂试验细胞分为：1正常对照组,平滑肌细胞完全培养基正常培养;2 DMSO（p38 MAPK抑制剂溶剂）对照组,0.01%DMSO处理;3 p38 MAPK抑制剂组,0.01%p38 MAPK抑制剂（20 mg/m L）处理。基因沉默试验细胞分为：1静态对照组,平滑肌细胞完全培养基正常培养;2阴性对照siRNA组,M ACSfectin试剂转染阴性对照siRNA 48 h;3 p38 M APK siRNA组,M ACSfectin试剂转染p38 M APK siRNA 48 h。采用qRT-PCR法检测Ⅰ型和Ⅲ型胶原mRNA表达;采用Western blotting法检测Ⅰ型和Ⅲ型胶原蛋白表达以及p38 MAPK转染效率;荧光显微镜观察siRNA转染效率。结果与正常对照组相比,DMSO对照组细胞Ⅰ型和Ⅲ型胶原mRNA和蛋白表达无明显变化（P〉0.05）,p38 MAPK抑制剂组细胞Ⅰ型和Ⅲ型胶原mRNA和蛋白表达显著降低（P〈0.05）。与静态对照组相比,阴性对照siRNA组细胞Ⅰ型和Ⅲ型胶原mRNA和蛋白表达无明显变化（P〉0.05）,p38 MAPK siRNA组细胞Ⅰ型和Ⅲ型胶原mRNA和蛋白表达显著降低（P〈0.05）。结论 p38 MAPK信号通路调控人主动脉平滑肌细胞Ⅰ型和Ⅲ型胶原的表达。

英文摘要：

Objective To investigate the effects of p38 MAPK on the expressions of type Ⅰ and type Ⅲ collagens in human aortic smooth muscle cells（ HASMCs）. Methods HASMCs were treated with inhibitors and siRNAs respectively. Inhibitor test cells were divided into 3 groups： ① normal control group（ cells were cultured in smooth muscle cell complete medium）; ② DMSO control group（ cells were treated with 0. 01% DMSO）; ③ p38 MAPK inhibitor group（ cells were treated with 0. 01% p38 MAPK inhibitor（ 20 mg / m L）. Gene silencing test cells were divided into 3groups： 1 static control group（ cells were cultured in smooth muscle cell complete medium）; 2 negative control siRNA group（ cells were transfected with negative control siRNAs for 48 h using MACSfectin reagent）; 3 p38 MAPK siRNA group（ cells were transfected with p38 MAPK siRNAs for 48 h using MACSfectin reagent）. The mRNA expressionsof type Ⅰ and Ⅲ collagens were detected by qRT-PCR,and the protein expressions were detected by Western blotting.The siRNAs transfection efficiency was observed with fluorescence microscope. Results Compared with the normal control group,the mRNA and protein expressions of type Ⅰ and Ⅲ collagens were not obviously changed in the DMSO control group（ P〉0. 05）,while the expressions decreased significantly in the p38 MAPK inhibitor group（ P〈0. 05）.Compared with the static control group,the mRNA and protein expressions of type Ⅰ and Ⅲ collagens were not obviously changed in the negative control siRNA group（ P〉0. 05）,while the expressions decreased significantly in the p38 MAPK siRNA group（ P〈0. 05）. Conclusion p38 MAPK signaling pathway regulates the expressions of type Ⅰ and type Ⅲ collagens in HASMCs.