Micromelalopha troglodyta (Graeser ) 是在中国的白杨的一个重要害虫。谷胱甘肽 S-transferases (GST ) 被知道为 M 的改编机制负责。troglodyta。这些活动和在 M 的谷胱甘肽 S-transferases 的运动常数。troglodyta 被学习。在谷胱甘肽 S-transferase 活动和运动特征的重要差别在 M 的五中间形态之中被观察。troglodyta 幼虫。而且,在由 24 个禁止者的五中间形态的谷胱甘肽 S-transferase 活动的抑制被进行。结果证明由 24 个禁止者的不同中间形态的 GST 活动的抑制是不同的。为在第一中间形态, chlorpyrifos , lambda-cyhalothrin , endosulfan , abamectin , fipronil 和 pyridaben 的 GST 活动,最好的禁止者被测试,并且为 GST ,并且为 GST 活动,在第二中间形态,鞣质的酸和橡黄素的活动是测试的大多数有势力禁止者,橡黄素, chlorpyrifos 和 lambda-cyhalothrin 的禁止的效果最高,并且为 GST ,在 第4 中间形态,橡黄素和 lambda-cyhalothrin 的活动是最好的禁止者,和禁止的效果我们的结果证明在不同中间形态的谷胱甘肽 S-transferases 是在 isozyme 作文并且这样不同的品质上在到禁止者的敏感不同。
Micromelalopha troglodyta (Graeser) is an important pest of poplar in China. Glutathione S-transferases (GSTs) are known to be responsible for adaptation mechanisms of M. troglodyta. The activities and kinetic constants of glutathione S-transferases in M. troglodyta were studied. Significant differences in glutathione S-transferase activity and kinetic characteristics were observed among five instars of M. troglodyta larvae. Furthermore, the inhibition of glutathione S-transferase activity in five instars by 24 inhibitors was conducted. The results show the inhibition of GST activity of different instars by 24 inhibitors was different. For GST activity in the 1st instar, chlorpyrifos, lambda-cyhalothrin, endosulfan, abamectin, fipronil and pyridaben were the best inhibitors tested, and for GST activity in the 2nd instar, tannic acid and quercetin were the most potent inhibitors tested, and for GST activity in the 3rd instar, the inhibitory effects of quercetin, chlorpyrifos and lambda-cyhalothrin were the highest, and for GST activity in the 4th instar, quercetin and lambda-cyhalothrin were the best inhibitors, and the inhibitory effect of phoxim was the highest for GST activity in the 5th instar. Our results show that glutathione S-transferases in different instars are qualitatively different in isozyme composition and thus different in sensitivity to inhibitors.