中文摘要：

目的 探讨皮肤黑素瘤和色素痣组织中趋化因子受体4（CXCR4）和趋化因子受体7（CXCR7）的表达及其与病理特征的相关性.方法 免疫组化法检测CXCR4、CXCR7在两个组（黑素瘤组及色素痣组）中的表达情况.结果 黑素瘤组与色素痣组免疫组化比较：CXCR4分别是20％（8/40）和50％（20/40）为（-）;12.5％（5/40）和42.5％（17/40）为（＋）;32.5％（13/40）和7.5％（3/40）为（＋＋）;35％（14/40）和0％（0/40）为（＋＋＋）;差异有统计学意义（均P＜ 0.05）.CXCR7分别是15％（6/40）和35％ （14/40）为（-）;10％（4/40）和62.5％ （25/40）为（＋）;22.5％（9/40）和2.5％（1/40）为（＋＋）;52.5％（21/40）和0为（＋＋＋）;差异有统计学意义（均P＜0.05）.CXCR4的表达水平在年龄、性别、Breslow厚度、溃疡情况、淋巴细胞浸润情况分组中差异无统计学意义（P＞0.05）;在发病部位、Clark分级、淋巴结转移分组中差异有统计学意义（P＜ 0.05）;CXCR7的表达水平在年龄、性别、溃疡情况、淋巴细胞浸润情况、淋巴结转移分组中差异无统计学意义（P＞0.05）;在发病部位、Breslow厚度、Clark分级分组中差异有统计学意义（P＜0.05）.结论 皮肤黑素瘤和色素痣组织标本中,CXCR4和CXCR7有不同程度的表达,CXCR4和CXCR7可能参与黑素瘤的发生发展.

英文摘要：

Objective To measure expressions of chemokine receptor 4 （CXCR4） and CXCR7 in cutaneous malignant melanoma （CMM） and pigmented nevus tissues,and to explore their correlations with pathological features.Methods Skin specimens were obtained from the lesions of 40 patients with CMM （CMM group） and 40 patients with pigmented nevus （pigmented nevus group）.An immunohistochemical study was performed to measure expressions of CXCR4 and CXCR7 in these specimens.Results In the CMM group,8 （20.0%） and 6 （15.0%） specimens were negative （-）,5 （12.5%） and 4 （10.0%） mildly positive （＋）,13 （32.5%） and 9 （22.5%） moderately positive （＋＋）,14 （35.0%） and 21 （52.2%） strongly positive （＋＋＋）,for CXCR4 and CXCR7 respectively.Of the pigmented nevus specimens,20 （50.0%） and 14 （35.0%） were negative （-）,17 （42.5%） and 25 （62.5%） mildly positive （＋）,3 （7.5%） and 1 （2.5%） moderately positive （＋＋）,for CXCR4 and CXCR7 respectively,but none was strongly positive （＋＋＋） for either of them.There were significant differences in the expressions of CXCR4 and CXCR7 between the CMM group and pigmented nevus group （U =317,256.5,respectively,both P 〈 0.01）.The expression of CXCR4 was significantly higher in non-acral than in acral melanomas （U =38,P 〈 0.05）,higher in Clark＇s level Ⅲ-V than in Ⅰ-Ⅱ melanomas （U =116,P 〈 0.05）,and higher in melanoma tissues from patients with lymphatic metastasis than in those without （U =16.5,P 〈 0.05）,but similar among patients of different age or gender,and among melanomas with different Breslow thickness,degrees of ulceration or lymphocytic infiltration （all P 〉 0.05）.No significant difference was observed in the expression of CXCR7 among patients of different age,gender,or among melanomas with different degrees of ulceration or lymphocytic infiltration,or between melanoma tissues from patients with lymphatic metastasis and those without （all P 〉 0.05?