中文摘要：

目的研究血浆半胱氨酸天冬氨酸蛋白酶-3（cysteine-asparticacidprotease-3，caspase-3）与炎症性肠病患者临床疾病活动性的相关性及对疾病的诊断价值。方法对克罗恩病患者、溃疡性结肠炎患者和正常对照者血浆中caspase-3的表达情况应用酶联免疫吸附试验（ELISA）进行分析。进一步分析血浆caspase-3浓度与内镜下疾病活动性的相关性。通过受试者工作特征曲线（receiver—operatingcharacteristic，ROC）观察炎症性肠病患者中血浆caspase-3水平的潜在临床及科研价值。数据处理使用GraphPadPrism5。结果克罗恩病患者血浆caspase-3水平显著高于正常对照组（P=0．0022），但溃疡性结肠炎患者血浆caspase-3水平与对照组无显著差异（P=0．6377）。caspase．3表达水平在区分克罗恩病患者和溃疡性结肠炎患者中诊断价值较低（AUC=0．6339，P=0．0039）。克罗恩病患者血浆caspase-3水平与内镜下疾病活动性无显著相关性（r=-0．089，P=0．5172）。结论克罗恩病患者血浆caspase-3表达水平增高，与溃疡性结肠炎及正常对照者存在差异。

英文摘要：

Objective To study the correlation of plasma cysteine- aspartic acid protease- 3 （ caspase- 3 ） with clinical disease activity in patients with inflammatory bowel disease and its diagnostic value. Methods The expression of plasma caspase-3 levels in patients with Crohn＇ s disease, ulcerative colitis and healthy controls were analyzed by enzyme-linked im- munosorbent assay （ELISA）. Furthermore, correlation of plasma caspase-3 level and endoscopic disease activity was also ana- lyzed. Receiver operating eharacteristic curve （ROC） was used to observe the potential clinical and research value of plasma caspase-3 in patients with inflammatory bowel disease. Data process was analyzed by GraphPad Prism 5. Results Plasma caspase-3 level was significantly higher in patients with Crohn＇ s disease than in healthy controls（P = 0. 0022）. However, plasma level of caspase- 3 showed no significant difference in patients with ulcerative colitis from healthy controls （ P = 0. 6377）. caspase-3 level showeed low diagnostic value in differentiating Crohn＇ s disease from healthy controls （AUC = 0. 6339 ,P = 0. 0039）. Plasma caspase-3 level was not significantly associated with endoscopic disease activity in patients with Crohn＇ s disease （r= - 0. 089,P = 0. 5172）. Conclusion Plasma caspase-3 expression increases in patients with Crohn＇ s disease. It is different from ulcerative colitis or healthy controls.