在大田条件下,采用叶面喷施的方法,研究了Mo、B、Mn和Zn 4种微量元素不同浓度(0 g/L、0.5 g/L、1 g/L和1.5 g/L)对人工种植乌拉尔甘草几种主要次生代谢成分积累的影响。结果表明,乌拉尔甘草根中甘草酸含量、甘草苷含量与Mn、Zn和Mo供应水平密切相关。其中,Mn、Zn元素的低、中、高浓度处理和Mo元素的中、高浓度处理可以显著提高乌拉尔甘草根中的甘草酸含量;Mo和Zn元素的低、中、高浓度处理及Mn元素的高浓度处理则可以显著提高乌拉尔甘草根中的甘草苷含量;Mn元素的中、高浓度处理及Zn元素的中浓度处理可以显著提高乌拉尔甘草根中的甘草多糖含量。4种微量元素的低、中、高浓度叶面喷施均可以显著提高乌拉尔甘草根中的总黄酮含量。由此认为,以上4种微量元素对改善人工种植乌拉尔甘草的品质具有一定的作用。
Effects of Molybdenum(Mo),Boron(B),Manganese(Mn) and Zinc(Zn) on accumulations of several secondary metabolites in Glycyrrhiza uralensis Fisch.roots were investigated under the field foliage spraying with 0 g/L,0.5 g/L,1 g/L and 1.5 g/L.The results show that the contents of glycyrrhizic acid and liquiritin in roots of Glycyrrhiza uralensis Fisch.are significantly related to Mn,Zn and Mo application levels.The glycyrrhizic acid contents in roots of Glycyrrhiza uralensis Fisch under the sprays of 0.5 g/L,1 g/L and 1.5 g/L Mn and Zn,1 g/L and 1.5 g/L Mo to the Glycyrrhiza uralensis Fisch.leaves are significantly increased.The additions of 0.5 g/L,1 g/L and 1.5 g/L Mo and Zn,1.5 g/L Mn induce significant increases of liquiritin contents in roots of Glycyrrhiza uralensis Fisch.In accordance with changes of the glycyrrhizic acid and liquiritin contents,the polysaccharide contents in roots of Glycyrrhiza uralensis Fisch.are significantly related to 1 g/L and 1.5 g/L Mn and 1 g/L Zn.The results also indicate that spraying Mo,B,Mn and Zn could increase total flavonoid content.It is concluded that external Molybdenum,Manganese and Zinc with moderate and high concentrations might have up-regulative effects on quality of Glycyrrhiza uralensis Fisch.