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中文摘要：
采用胶束电动毛细管色谱法（MECC）对硫酸多粘菌素E药物中的主要成分多粘菌素E1和E2进行了分离,并测定了多粘菌素E1、E2的含量。分别考察了电泳电压、表面活性剂种类、Brij-35（月桂醇聚氧乙烯醚）浓度、乙腈含量、磷酸盐缓冲液的pH值、氯化钠浓度等实验参数对实验结果的影响,从而确定了最佳的分离条件：电泳电压为10kV,运行缓冲液为含有30mmol/L Brij-35、5%（体积分数）乙腈、0.167mol/L氯化钠的磷酸二氢钠缓冲液（0.01mol/L,pH4.1）。在优化的实验条件下,E1和E2得到了较好的分离,分离度达到1.94。以多粘菌素E1为例,柱效和峰面积的日间及日内测定的相对标准偏差（RSD）均小于5%。E1和E2在硫酸多粘菌素E药物中的含量分别为67%和32%。该方法简便、快速、准确、重现性好。
英文摘要：
A method of micellar electrokinetic chromatography capillary （MECC） has been established for separating polymyxins E1 and E2 in polymyxin E sulfate and determining the contents of E1 and E2. Several factors including the running voltage, the type of surfactant, concentrations of Brij-35 （polyoxyethyleneglycol dodecyl ether）, NaCl solution and acetonitrile, pH of phosphate were investigated. Under the optimum conditions （ 10 kV running voltage, phosphate buffer solution （0.01 mol/L, pH 4. 1 ） containing 30 mmol/L Brij-35, 5% （v/v） acetonitrile, 0. 167 mol/L NaCl）, E1 and E2 were separated with the resolution of 1. 94. The contents of E1 and E2 in polymyxin E sulfate were 67% and 32%, respectively. As an example, the relative standard deviations of the intra-assay and inter-assay of polymyxin E1 on the plate number and peak area were less than 5%. The method is simple, rapid, accurate, and reproducible.
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