中文摘要：

为优化电激活和胚胎培养的相关技术体系，研究电激活参数和培养液对猪卵母细胞孤雌胚胎发育的影响。结果表明：在脉冲电压为150v（以每1mm电激槽宽计，下同）、脉冲持续时间为100μS、脉冲1次的电激活条件下，猪卵母细胞孤雌激活的效果较好；在脉冲持续时间和脉冲次数恒定的条件下，脉冲电压150V组的囊胚率显著高于100V组和125V组（P〈0．05），达46．3％；在脉冲电压和脉冲次数恒定的条件下，脉冲持续时间100us的囊胚率显著高于75、125μs组（P〈0．05），达50．5％；在脉冲电压和脉冲持续时间恒定的条件下，1次脉冲的卵裂率和囊胚率显著高于2次脉冲组（P〈0．05），分别达82．7％、50．3％；PZM3和NCSU23培养液对猪孤雌激活胚胎发育的影响差异不显著（P〉0．05），但PZM3培养液组的卵裂率、囊胚率和囊胚细胞数均略高于NCSU23组。

英文摘要：

In order to optimize electrical activation and oocytes culture condition, the effect of different electrical activation parameters and culture media on parthenogenetic activation of porcine oocytes was investigated. The result indicated that one-time pulse electrical activation under pulse voltage of 150 V/mm and pulse duration of 100μs was the best condition for parthenogenesis of porcine oocytes. The blastocyst rate in the group treated with a pulse voltage of 150 V/mm reached up to 46.3%, which was significantly higher than that in the groups treated with pulse voltages of 100 V/mm and 125 V/mm respectively under the same condition of pulse duration and pulse times （P〈0.05）. And the blastocyst rate in the group treated with pulse duration of 100μs reached up to 50.5%, which was significantly higher than that in the other two groups treated with pulse duration of 75μs and 125μs respectively under the same condition of pulse voltage and pulse times （P〈0.05）. The cleavage rate and the blastocyst rate in the group treated with one-time pulse reached up to 82.7% and 50.3% respectively, which was significantly higher than that in the group treated with two-time pulse under the same condition of pulse voltage and pulse duration （P〈0.05）. The activated parthenogenetical development of porcine oocytes in groups cultured in media PZM3 and NCSU23 respectively showed no significant difference （P 〉 0.05）, but cleavage rate, blastocyst rate and blastocyst cell number in the group cultured in medium PZM3 were a little higher than that in the group cultured in medium NCSU23.