中文摘要：

以版纳微型猪近交系妊娠47d的胎儿为材料,采用胰蛋白酶消化法消化培养胎儿成纤维细胞,对其进行细胞形态观察、细胞冻存前和复苏后存活率测定、生长曲线绘制和染色体核型分析等生物学特征检测.结果表明,培养的版纳微型猪近交系胎儿成纤维细胞呈典型的成纤维细胞形态;细胞冻存前和复苏后的存活率分别为98.06%和92.12%;生长曲线呈＂S＂形,倍增时间为36h;对所制备染色体核型进行分析,显示2n=38,XY,并在体外培养14个代次后仍能保持正常核型.

英文摘要：

Useing a 47 d fetus of Banna minipig inbred line as materials,fetal fibroblast cells were dispersed in vitro with trypsinnization and then subcultured.Observations on morphology,determination of viability,drawing of dynamic growth,and analysis of karyotype were carried out.The results showed that the cell was morphologically consistent with fibroblasts,cell viability was 98.06% before freezing and 92.12% after thawing,the growth curves was sigmoidal with population doubling time（PDT）of cells was approximately 36 h,karyotyping indicated a total chromosomes number 2n=38,sex chromosomes were XY,and the karyotype is normal afrer cultured up to passage 14 in vitro.