中文摘要：

构建重组质粒pc DNA3.1-DOK2并转染胃癌细胞BGC823,采用平板集落形成、CCK8法和软琼脂克隆形成实验检测过表达DOK2基因对BGC823生物学行为的影响,并采用免疫组织化学法检测116例胃癌组织中DOK2蛋白的表达,分析其与胃癌临床病理学特征及预后的关系。结果显示,过表达的DOK2基因对BGC823细胞增殖有显著抑制作用。在所有胃癌组织中,DOK2蛋白低表达占62.93%,且其与胃癌浸润深度、淋巴结转移以及分化程度密切相关;DOK2蛋白的表达、胃癌浸润深度、淋巴结转移、远处转移以及分化程度是影响患者预后生存时间的重要因素。

英文摘要：

In this research, the recombinant plasmid pcDNA3.1-DOK2 was constructed and transfected into BGC823 cells. We evaluated the influence of over-expression of DOK2 on gastric cancer cells biological behavior by CCK8, soft agar and colony formation assay. The expression of DOK2 protein was detected by imrnunohistochemistry in 116 cases of gastric carcinoma, and its relationship with clinicopathological characteristics and prognosis of gastric cancer was analyzed. The results showed that over-expression of DOK2 significantly inhibited the growth, proliferation and colony of BGC823 cells. Immunohistochemistry showed the low expression of DOK2 protein in gastric cancer accounted for 62.93%, and DOK2 protein expression had a significant inverse correlation with depth of tumor invasion, lymph node metastasis and tumor differentiation degree; DOK2 protein expression, depth of tumor invasion, lymph node metastasis, distant metastasis and tumor differentiation degree were important factors affecting the prognosis of patients survival.