中文摘要：

为了探究位于IGF1R基因3’UTR与前列腺癌（prostatecancer，PCa）~fH关的多态性位点rs2016347通过改变与miRNA结合影响PCa的风险，本研究用miRbase预测靶向miRNA，然后用热力学模型方法计算结合能情况，最后用双荧光素酶报告基因技术对HEK293T在体外检测改变rs2016347位点对靶向miRNA结合的影响。miRbase预测结果显示，hsa—miR-3175与IGF1R的结合区位于多态性位点rs2016347。热力学模型方法计算结果表明，相对位点G，hsa-miR3175与IGF1R的3’UTR端在rs2016347位点T上更能有效结合。双荧光素酶报告基因的检测结果显示，hsa—miR-3175能与带有rs2016347等位位点（G或T1IGF1R3’UTR结合，hsa—miR-3175与等位位点T的结合更稳定，hsa-miR-3175与IGF1R基因的结合起到稳定IGF1R基因表达的作用。多态性位点rs2016347等位位点T在PCa的发展中风险性更大。

英文摘要：

To study the polymorphism rs2016347 in IGF1R 3 untranslated region （UTR） that is associated with prostate cancer （PCa） and affected disease risk by altering miRNA binding, the targeted miRNA was predicted through miRbase, and the thermodynamic model method was applied to calculate the binding energy. Furthermore, dual luciferase reporter assay was used to verify whether binding affinity of miRNA was influenced through altering allele of rs2016347 in HEK293T cell in vitro. Prediction of miRbase demonstrated that binding region of hsa-miR-3175 and IGF1R was located in the potymorphism rs2016347. Calculation results of thermodynamic model method indicated that T allele of rs2016347 would generate a stronger binding of hsa-miR3175 on IGF1R 3＇UTR compared with the G allele. Dual luciferase reporter assay indicated hsa-miR-3175 binding to IGF1R 3＇ UTR contained G allele or T allele of rs2016347, and showed a stronger binding with T allele. The binding of hsa-miR-3175 and IGF 1R gene played a role in stabilizing the IGF1R gene expression. Polymorphism rs2016347 with T allele showed a greater risk in the progression of PCa.