中文摘要：

目的：比较海藻酸钠加明胶、胶原、琼脂糖、基质胶等4种支架材料培养前列腺癌PC3细胞效果.方法：将前列腺癌PC3细胞接种到海藻酸钠加明胶、胶原、琼脂糖、基质胶等4种支架材料中进行三维培养,然后采用实时荧光定量RT-PCR方法检测MMP9、MMP2、FN1、LAMA5、LAMC2、NKX3 1等6个前列腺癌相关基因在上述4种支架材料培养的PC3细胞的表达情况.结果：MMP9、MMP2在有支架材料培养的PC3细胞中表达高于无支架材料培养的PC3细胞;LAMC2、MMP9、MMP2在胶原组中上调,MMP9、MMP2在海藻酸钠加明胶组与琼脂糖原组中上调,基质胶组中只有MMP2上调.结论：①采用支架材料的三维培养体系优于不用支架材料的二维培养体系.②胶原为体外前列腺癌PC3细胞三维培养的最优支架材料.

英文摘要：

Objective：To evaluate the expression level of prostate cancer-related genes in prostate cancer PC3 cells which were cultured in the mixture of alginate and gelatin,collagen,agarose,Matrigel scaffolds.Methods：Human prostate cancer PC3 cells were seeded into the mixture of alginate and gelatin,collagen,agarose,Matrigel scaffolds for three-dimensional culture,and real-time fluorescent quantitative RT-PCR method for detecting the expression level of 6 prostate cancer related genes MMP9,MMP2,FN1,LAMA5,LAMC2,NKX3-1in PC3 cells which were cultured in the four scaffolds.Results：The expression level of MMP9 and MMP2 was significantly higher in PC3 cells with scaffold than in PC3 cells without scaffolds.The expression level of LAMC2 increased in collagen group,decreased in agarose group.The expression level of MMP9 and MMP2 increased in the mixture of alginate and gelatin and collagen group,and in both groups the expression level of the results of increased MMP2 was consistent.In Matrigel group,only the expression level of MMP2 was increased.Conclusion：The three-dimensional culture system scaffold is superior to two-dimensional culture system.Collagen is the best scaffold for culturing human prostate cancer PC3 cells.