中文摘要：

目的检测胃癌患者血清外泌体（exosome）中抗分化非编码RNA（DANCR）的表达水平并分析其临床应用价值。方法采用实时荧光定量RT-PCR检测胃癌细胞培养上清和胃癌患者血清exosome中DANCR表达水平,分析其与临床病理资料的相关性,绘制ROC曲线评价其诊断效能。结果 DANCR在人胃癌细胞系HGC-27中表达水平（4.43±0.37）明显高于胃黏膜上皮细胞（1.01±0.01）,差异有统计学意义（P〈0.05）。HGC-27细胞培养上清exosome中DANCR表达水平（3.06±0.14）明显高于GES-1细胞（1.01±0.20）,差异有统计学意义（P〈0.05）。DANCR在胃癌患者血清exosome中的平均含量[5.060（1.380,22.785）]较胃良性疾病患者[0.535（0.340,1.380）]和体检健康者[1.200（0.605,1.655）]明显升高（Z分别为-3.409,-4.229,P均〈0.01）,且与肿瘤大小、TNM分期以及淋巴结转移相关。胃良性疾病患者与体检健康者相比,血清exosome中DANCR含量差异无统计学意义（Z=-1.308,P〉0.05）。胃癌患者血清exosome中DANCR的ROC曲线下面积（AUCROC）为0.777,95%可信区间（CI）为0.678~0.876,cut-off值2.50,敏感性为68.6%,特异性为84.4%。结论胃癌患者血清exosome中DANCR表达水平升高,有望成为胃癌诊断的新指标。

英文摘要：

Objective To detect the expression levels of anti-differentiation antagonizing noncoding RNA （DANCR） in exosomes from serum of gastric cancer patients and evaluate its clinical application. Methods The expression levels of DANCR in exosomes derived from the culture supernatants of gastric cancer cell lines and the serum samples of gastric cancer patients were detected by using realtime qRT-PCR. The correlations between DANCR levels and the clinicopathological parameters were analyzed. A receiver operating characteristic（ROC） curve was drawn to evaluate the diagnostic efficiency of DANCR. Results The expression level of DANCR in HGC-27 cell（4.43 ±0.37） was significantly higher than that in GES-1 cells （1.01±0.01 ）（P 〈 0.05 ）. The expression levels of DANCR in exosomes from the supernatants of HGC-27 cells（3.06 ±0.14） were significantly higher than that from GES-I cells（ 1. 01 ± 0.20） （P 〈0.05 ）. The expression levels of DANCR in exosomes from the serum samples of gastric cancer patients [ 5. 060 （1. 380, 22.785 ） ] were significandy higher than that in gastric benign disease [ 0. 535 （ 0. 340, 1. 380） ] （ Z = - 3. 409, P 〈 0.01 ） and healthy controls [ 1. 200（0.605, 1. 655） ] （Z = -4.229, P 〈0.01 ）. There was no significant difference in the expression levels of serum exoso- mal DANCR between gastric benign disease group and healthy control group （Z = -1. 308, P 〉 0.05 ）. In addition, the expression levels of DANCR in gastric cancer patients were related to tumor size, TNA stage and lymph node metastasis. The area under the ROC curve of serum exosomal DANCR expression levels was 0. 777 in the diagnosis of gastric cancer, the 95% confidence interval was 0. 678 to 0. 876 and the cutoff value was 2.50. The sensitivity was 68.6% and the specificity was 84.4%. Conclusion The expression level of serum exosomal DANCR may increase in gastric cancer patients and could be served as a novel marker for the diagnosis of gastric cancer.