以猕猴桃籽为原料,采用水酶法提取猕猴桃籽油。在单因素试验的基础上,采用二次正交旋转组合试验对猕猴桃籽油的提取工艺进行优化,建立了各主要影响因素与猕猴桃籽出油率之间的回归模型。试验结果表明:各因素对猕猴桃籽出油率影响的顺序为液料比〉酶解pH〉酶添加量〉酶解温度,水酶法提取猕猴桃籽油的最优工艺参数为液料比10,酶解温度50℃,酶添加量2.5%,酶解pH9.0。在此条件下,提取二次总出油率为27.17%,萃取率达86.75%。所建立的数学回归模型能够较准确预测猕猴桃籽油的出油率。
Taking kiwi -fruit seed as raw material, the aqueous enzymatic extraction technology was used to extract kiwi -fruit seed oil. Based on single factor experiments, extraction conditions of kiwi -fruit seed oil were optimized through quadratic regression orthogonal design, and model was established to express the relationship between oil yield and the main affecting factors. Results showed: the factor order on extraction yield of kiwi - fruit seed oil from strong to weak was liquid - solid ratio 〉 enzymolysis pH 〉 dosage of Alcalase 〉 enzymolysis temperature. The optimal extraction parameters were :liquid - solid ratio 10, enzymolysis temperature 50 ℃, dosage of Alcalase 2.5 %, and enzymolysis pH 9.0. Under such conditions ,the total oil yield after extraction twice was 27.17% ,extraction rate reached as high as 86.75%. The established regression mathematical model can predict the oil yield of kiwi - fruit seed accurately.