中文摘要：

目的：探讨纳米金如何阻断VEGF165的信号传导，抑制裸鼠移植肝癌血管生成。方法：无血清培养人脐静脉血管内皮细胞（HUVEC），加入血管内皮生长因子165（VEGF165），再加入不同浓度纳米金，作用5min，采用Western blot方法测定磷酸化PLC-γ1蛋白、6周龄Balb／c裸鼠20只，从裸鼠右腋皮下注入H22细胞。第5天，肿瘤形成约8mm大小，随机分为两组：实验组从肿瘤周围及瘤内注入纳米金，每天1次，连续8天；对照组用生理盐水处理。处死裸鼠时测量肿瘤体积及重量，免疫组化染色并计算肿瘤组织微血管密度。结果：VEGF165浓度不变（10μg，L），随着纳米金溶液浓度的增加（125、250、500nmol／L），纳米金抑制PLC-γ1磷酸化越来越明显。肝癌组织内微血管密度分别为，实验组14．27±1．08，对照组23．52±1．36，说明纳米金明显抑制了肝癌血管生成（P〈0．01）。结论：纳米金阻断VEGF165的信号传导，明显抑制裸鼠肝癌血管生成。

英文摘要：

Objective： To investigate how nanogold blocks the signal transduction of VEGF165 （vascular endothelial growth factor 165） and inhibits angiogenesis and growth of transplanted liver cancer in nude mice. Methods： Human umbilical vein endothelial cells （HUVEC） were serum-starved for 24 hours and treated with VEGF165 and then with different concentrations of nanogold for five minutes. Phosphorylated PLC-γ1 protein was detected with Western blot. H22 cells were injected into the subcutaneous right axillary vein in 20 Balb/c nude mice. The size of the transplanted tumor was about 8 mm after five days. The nude mice were divided in- to the experimental group and the control group. In the experimental group, nanogold was injected into the tumor once a day for a period of 8 days. The mice in the control group were treated with saline. The mice were sacrificed and the size and weight of the tumors were measured. Microvascular density （MVD） of each tumor was determined by immunohistochemical staining. Results： When VEGF165 concentration was unchanged （10μg/L）, its inhibitory effect on PLC-γ1 phosphorylation was increasingly obvious as nanogold concentration increased （125,250, and 500 nmol/L）. The MVD in liver cancer tissue was 14.27±1.08 in the nanogold treated group and 23.52±1.36 in the control group, with a significant difference （P〈0.01）. Conclusion： Nanogold inhibits angiogenesis in liver cancer by blocking the signaling of VEGF165.