中文摘要：

目的观察纳米金（GNP）对人微血管周细胞增殖和G蛋白信号调节蛋白5（RGS5）表达的影响。方法制备浓度为50nmol／L直径分别为25、50、100nm的GNP溶液；体外培养人微血管周细胞株HBVP-1200；光学显微镜观察GNP作用周细胞、在细胞中沉积；噻唑蓝（MTT）比色法检测周细胞增殖；原子力显微镜（AFM）观察周细胞表面形貌；Westernblot检测周细胞RGS5蛋白表达。结果（1）不同组间的GNP（25、50、100nm）及对照组，其周细胞增殖率分别为：（147．9±5．9）％、（121．7±3．4）％、（107．6±2．1）％及（100．0±0．0）％；粒径越小，周细胞增殖率越高（P〈0．05）。（2）AFM观察到粒径越小的GNP处理后，周细胞表面形貌变化越明显。主要表现在细胞膜内陷、细胞表面出现较大孔洞，有细胞内吞现象。（3）Westernblot检测到粒径越小的GNP，抑制RGS5蛋白表达越明显。结论GNP促进人微血管周细胞增殖，抑制周细胞RGS5蛋白表达；并且GNP粒径越小，对周细胞影响越明显。当GNP粒径达到100nm时，对周细胞增殖和抑制RGS5蛋白表达几乎无作用。

英文摘要：

Objective To observe the effects of nangold~（GNP） on proliferation and the expres- sion of regulator of G-protein signaling5 （ RGS5 ） of human microvascular pericytes. Methods The GNP solutions of 60 nmol/L with diameters of 25, 50 and 100 nm were prepared. HBVP-1200 cells were cultured in vitro. The methyl thiazolium tetrazolium （MTT） was used to detect the effects of different diameters of GNP on proliferation of pericytes. Atomic force microscope （AFM） was used to observe morphological chan- ges of pericytes. The expression of RGS5 protein was detected by using Western blotting. Results GNP could enhance the proliferation of human mierovascular pericytes. After treatment with different diameters of GNP （25, 50, and 100 nm）, the proliferation rate of pericytes was （ 147. 9 ± 5.9 ） %, （ 121.7±3.4）% and （ 107.6± 2. 1 ）% respectively. When the diameter of GNP was smaller, the proliferation rate was increased significantly （P 〈 0. 05）. Under AFM, the morphological changes of pericytes treated with GNP were ob- served, including invagination, obvious shrinked cell membrane and much rougher surface. The protein ex- pression of RGS5 was reduced when the GNP was given 24 h later, especially when the diameter of GNP was 25 nm. Conclusion GNP could enhance the proliferation of human microvascular pericytes, and restrain the RGS5 protein expression. The smaller the diameter of GNP, the stronger effects of GNP on the pericytes.