中文摘要：

Myostatin基因属于TGF-β超家族,是动物骨骼肌生长发育的负调控因子。在已克隆大黄鱼Myostatin-1基因编码序列的基础上,采用基因组步移的方法,首次克隆到约1kbp的大黄鱼Myostatin-1启动子序列。该启动子序列与已报道的金鲷和大口黑鲈相应序列的相似度分别为90%和75%。该序列具有1个TATAbox、1个CAATbox、5个E-box位点,此外还具有MyoD,USF,MEF2,SP1,NF-Y等多种转录因子结合位点。

英文摘要：

Myostatin is a member of the transforming growth factor-β superfamily,which functions as a negative regulator for the development and growth of skeletal muscle in animal. Based on the cloned myostatin-1 gene of Larimichthys crocea,the promoter sequence of L. crocea myostatin-1 gene was cloned by genomic walking. The sequencing results indicate that it shares 90% and 75% homology identity with the corresponding reported sequences of Sparus aurata and Micropterus almoides,respectively. Bioinformatic analysis showed that myostatin-1 gene contains one consensus sequences of TATA box,a CAAT box and five putative E-boxes known as the binding sites to myogenic basic helix-loop-helix transcription factors. In addition,it also possesses many transcription factor binding sites,such as MyoD,USF,MEF2,SP1 and NF-Y. These results are expected to be the basis for further studies on the regulation and expression of this gene.