目的:研究胞外不同浓度的镁离子对SD成年大鼠心肌细胞钙瞬变的影响。方法:采用激光共聚焦显微镜系统同步配合阈上电刺激探测心肌细胞钙瞬变。结果:胞外低浓度的镁离子(0 mmol /L,0.5 mmol /L; 正常镁离子浓度为1 mmol/L)可以升高钙瞬变的峰值(P〈0.05),但不影响钙瞬变的持续时间(以钙瞬变的半高宽表示)(P〉0.05);胞外高浓度的镁离子(2.5 mmol /L,5 mmol /L,10 mmol /L)均可抑制钙瞬变的峰值(P〈0.05);其中5 mmol/L和10 mmol/L的胞外镁还能延长钙瞬变的持续时间(P〈0.05)。结论:正常情况下镁对心肌细胞钙瞬变有抑制作用。
Purpose: To investigate effects of different concentrations of extracellular magnesium ions on calcium transients in cardiac cells of SD adult rats. Methods: Cardiac calcium transients were detected by the LSM510 META confocal microscopic system, working synchronically with local field stimulation. Results: Low concentrations of extracellular magnesium ions(0 mmol /L,0.5 mmol /L; normal concentration is 1 mmol/L) increase the peak of calcium transients (P〈0.05), without affecting the duration of calcium release(P〉0.05); cardiac calcium transients were inhibited by higher concentrations of extracellular magnesium ions (2.5 mmol /L,5 mmol /L,10 mmol /L) (P〈0.05), and the duration of calcium transients were prolonged by extracellular solution containing 5 mmol /L or 10 mmol /L magnesium ion(P〈0.05).Conclusion: Magnesium ion can inhibit cardiac calcium transients.