应用DNA序列研究昆虫的系统发育和进化规律已成为当今分子系统学研究的热点,结合国内外最新研究成果对线粒体基因和核基因在蝶类分子系统学中的应用进行系统归纳和总结。主要从线粒体基因(常用分子标记如:16SrDNA、Cytb、COI、COII、ND5等)以及线粒体基因组、核基因[常用分子标记如:28SrDNA、视蛋白(OPSl)基因、周期(Period)基因、丙糖磷酸异构酶(Tpi)和甘露糖磷酸异构酶(Mpi)基因、延长因子(EF—la)基因、无翅基因(Wingless)等]、多基因联合等3方面阐述蝶类不同分类阶元系统发育研究的进展。线粒体基因与核基因、多基因联合分析已成为当前蝶类分类和系统关系研究的主要手段,未来更多基因片段将被用于蝶类分子系统学研究,更多的蝶类线粒体基因组全序列将被测序;最后,也探讨了目前分子系统学研究中存在的一些问题。
Application of DNA sequences in phylogentic relationships and evolutionary history of in sects has become a hotspot in molecular systematics. Combined with the latest studies, the recent pro gresses in phylogeny of butterflies both in China and abroad are reviewed from three aspects, specific ally, molecular studies on systematic of butterflies applying the mtDNA(mainly including. 16S rD NA, Cyt b, CO Ⅰ, COⅡand ND5 ), the complete mitochondrial genome,nuclear genes (mainly in cluding.. 28S rDNA, OPS1, Period, Tpi, Mpi, EFI, and Wingless) and a combination of sequences analyses. The application of mtDNA genes combining with nuclear genes has become the major meth od to address the problems of taxonomiy and phylogenetic relationships of butterflies. More gene se quences will be utilized in molecular systematics of butterflies and more complete mitochondrial ge nome of butterflies will be determined in the future. Finally, the deficiency and problems in molecular systematics are also discussed in this paper.