中文摘要：

目的用人源性神经母细胞瘤细胞株（SH-SY5Y）研究环境因素铝和遗传因素ApoEε4等位基因单独及联合作用对细胞磷酸化tau蛋白和Aβ表达的影响。方法选取SH-SY5Y细胞进行AlCl3染毒和ApoEε4转染处理，实验分组分为对照组、400 μmol/L AlCl3组、ApoEε4转染组、400 μmol/LAlCl3＋ApoEε4转染组。AlCl3染毒（或）和ApoEε4转染48 h后，采用CCK-8试剂盒测定细胞活力；用ELISA试剂盒测定细胞总tau蛋白、p-tau181、p-tau231、p-tau396和Aβ。结果400 μmol/L AlCl3组、ApoEε4转染组、400 μmol/L AlCl3＋ApoEε4转染组细胞活力明显著低于对照组，差异有统计学意义（P〈0.05），且AlCl3和ApoEε4存在交互作用（P〈0.05），表现为协同效应。tau蛋白和Aβ测试结果显示400 μmol/L AlCl3组、ApoEε4转染组、400 μmol/L AlCl3＋ApoEε4转染组tau5、p-tau181、231、396、Aβ含量均明显高于对照组，差异有统计学意义（P〈0.05），且AlCl3和ApoE ε4存在交互作用（P〈0.05），表现为协同效应。结论环境因素铝和遗传因素ApoE ε4等位基因均可促使细胞总tau、磷酸化tau蛋白表达升高，Aβ沉积增多，且两因素共同存在具有协同效应。

英文摘要：

ObjectiveTo investigate the expression of phosphorylated tau （p-tau） and Aβ in SH-SY5Y cells induced by aluminum or/and ApoE ε4 allele, and study the interaction between aluminum and ApoE ε4 allele.MethodsSH-SY5Y cells were assigned to control group, 400 μmol/L AlCl3 group, ApoEε4 transfected group and 400 μmol/L AlCl3 with Apo Eε4 transfected group. The cell viability was measured by CCK-8 assay; the expressions of p-tau and Aβ was determined with ELISA Kit after AlCl3 exposure or or/and ApoE ε4 transfection.ResultsThe viability of cells exposed to 400 μmol/L AlCl3 or/and ApoE ε4 transfected were significantly lower than that of controls （P〈0.05） . The expressions of total tau, tau-181, tau-231, tau-396 and Aβ in 400 μmol/L Al3＋ or/and ApoE ε4 transfected exposed cells showed significantly higher than those of controls （P〈0.05） . Based on the factorial design, a significant interaction exists, and there is a synergistic effect between AlCl3 and ApoE ε4 （P〈0.05） .ConclusionAluminum and ApoE ε4 allele could increase expression of p-tau and Aβ deposition; there was a synergistic interaction between aluminum and ApoE ε4 allele on cell death, tau phosphorylation and Aβ deposition of SH-SY5Y.