中文摘要：

目的探讨三氯化铝对SH-SY5Y细胞及tau蛋白异常磷酸化水平的影响。方法选取SH—SY5Y细胞进行三氯化铝染毒，实验分为对照组、200、400和800p．mol／LAl3＋染毒组，染毒48h后观察细胞形态学变化，采用CCK-8法测定细胞活性，并提取细胞蛋白，采用蛋白免疫印迹法（Western blot）检测细胞蛋白P—tau181、231、262、396和tau5的表达情况。结果随着染铝浓度增加细胞数目减少，突触回缩，细胞活性测定结果显示，800μmol／LAl3＋组细胞活性明显低于对照组，差异有统计学意义（P〈0．05）；Western-Blot结果显示200、400和800μmol／LAl3＋组P-tau181、231、396和tau5蛋白的表达均明显高于对照组，差异有统计学意义（P〈0．05），800μmol／LAl3＋组P-tau262蛋白表达明显高于对照组，差异亦有统计学意义（P〈0．05）。结论在本实验条件下，三氯化铝对SH-SY5Y细胞有毒性作用，低浓度就可引起tau181、231、396和tau5表达异常。

英文摘要：

Objective To investigate the effect of aluminum trichloride on the abnormal phosphorylation of tau protein in SH-SY5Y cells. Methods SH-SY5Y cells were assigned to control group and aluminum trichloride exposure groups （200, 400, and 800 μmol/L Al3＋）. The cell morphology was observed after 48 hours of exposure; the cell viability was measured by CCK-8 assay; total protein was extracted from the （：ells, and the expression of phospho-tau （p-tau） 181, 231,262, and 396 and tau 5 was measured by Western blot. Results As the Al3＋ concentration rose, the number of living SH-SY5Y cells decreased, and the synapses of the （：ells retracted. The viability of cells exposed to 800 p, mol/L Al3＋ was significantly lower than that of the control group （P〈0.05）. The 200, 400, and 800 μmol/L Al3＋ exposure groups showed significantly higher expression of p-tan 181, 231, and 396 and tan 5 than the control group （P〈0.05）, and the 800μmol/L Al3＋ exposure group showed significantly higher expression of p-tau 262 than the control group （P〈0.05）. Conclusion Under the present experimental conditions, aluminum tfiehloride has toxic effect on SH-SY5Y cells and can lead to abnormal expression of p-tau 181, 231, and 396 and tau 5 at low Al3＋ concentration.