中文摘要：

目的探讨microRNA-4731-3p（miR-4731-3p）在肺癌血浆中的表达及其临床意义。方法采用实时荧光定量PCR（qRT-PCR）方法检测40例肺癌患者和40例健康人（作为对照）血浆miR-4731-3p的表达水平,分析其与临床病理参数和预后的相关性。绘制受试者工作特征曲线（ROC）,评价血浆miR-4731-3p表达水平对原发性肺癌的诊断价值。结果肺癌血浆中miR-4731-3p的表达水平（0.11±0.05）显著高于健康人（0.05±0.03）（P〈0.001）;miR-4731-3p表达水平与癌胚抗原（CEA）正相关（P〈0.05）,与患者性别、年龄、吸烟、病理类型、肿瘤大小、临床分期、远处转移均无明显相关性（P〉0.05）。血浆miR-4731-3p在ROC曲线下面积（AUC）为0.835（95%CI=0.750~0.921）。当miR-4731-3p临界值取0.048时,对肺癌诊断的灵敏度为0.950,特异度为0.575。KaplanMeier分析显示肺癌血浆中miR-4731-3p低表达者生存时间明显高于高表达组（P〈0.05）。结论血浆miR-4731-3p在肺癌血浆中表达上调,可作为肺癌诊断及预后判断的潜在分子标志物。

英文摘要：

Objective To investigate the expression of microRNA-4731-3p（ miR-4731-3p） in the plasma of patients with lung cancer and to determine the correlation between miR-4731-3p level and clinical parameters. Methods Quantitative real-time PCR（ qRT-PCR） was used to examine the plasma level of miR-4731-3p in 40 patients with lung cancer and 40 healthy persons（ control）.The correlation between the plasma miR-4731-3p level and clinicopathological features and prognosis were investigated.Receiver operating characteristic（ ROC） curve were drawn to evaluate the diagnosis value of miR-4731-3p expression in plasma for lung cancer. Results The plasma miR-4731-3p was significantly up-regulated in patients with lung cancer as compared with healthy subjects. The expression of miR-4731-3p was associated with carcino-embryonic antigen（ CEA）（ P〈0.05）,while it was not associated with gender,age,smoking,pathology type,tumor size,clinical stage and distant metastasis（ P〉0.05）.The area under the ROC curve（ AUC） according to the miR-4731-3p expression in plasma was 0.835（ 95% CI = 0.750 ~ 0.921）,when the critical value was 0.048.The sensitivity for lung cancer diagnosis and specific degrees were 0.950 and 0.575 respectively.The expression of miR-4731-3p was correlated with overall survival of lung cancer（ P〈0.05）. Conclusion The expression of miR-4731-3p is up-regulated in tumor,and it may be a potential biomarker for the diagnosis and prognosis evaluation of lung cancer.