中文摘要：

目的 探讨新生大鼠脑室周围-脑室内出血（PIVH）后铁蓄积及铁代谢相关蛋白的表达变化. 方法 40只健康新生第7天SD大鼠按随机数字表法分为胶原酶出血组和对照组,每组各20只,其中胶原酶出血组利用立体定向技术将Ⅶ型胶原酶用微量注射器精确注入大鼠右侧基底节诱导成PIVH模型,对照组注射等量生理盐水.制模后1d通过干湿重法测定2组大鼠脑组织含水量,制模后1、3、28 d通过增强普鲁士蓝染色观察铁沉积情况,制模后1、3、28 d通过免疫组化染色观察及制模后1d通过Western blotting检测血红素加氧酶1（HO-1）和铁蛋白轻链、重链的表达情况. 结果 新生大鼠PIVH后1d右侧大脑半球脑组织含水量较对照组明显升高,差异有统计学意义（P＜0.05）.制模后1、3、28 d增强普鲁士蓝染色显示胶原酶出血组血肿周围可见大量铁沉积,脑室壁周围组织内可见大量铁染色阳性细胞,而对照组无铁沉积表现;免疫组化染色显示胶原酶出血组右侧基底节及脑室周围可见大量HO-1阳性细胞表达及铁蛋白阳性细胞表达,而对照组仅可见极少量阳性细胞.制模后1d Western blotting检测显示胶原酶出血组HO-1含量及铁蛋白重链、铁蛋白轻链含量与对照组相比明显升高,差异有统计学意义（P＜0.05）. 结论 铁参与了PIVH的脑损伤过程,其有可能成为新生儿PIVH的治疗靶点.

英文摘要：

Objective To investigate the iron accumulation and iron-handling proteins expressions （heme oxygenase-1 [HO-1] and ferritin） in new-born rats after periventricular-intraventricular hemorrhage （PIVH）.Methods Forty 7-day-old SD rats were randomized into control group and experimental group （n=20）.PIVH was induced by intraparenchyrnal infusion of bacterial collagenase into the right basal ganglia; equivalent saline was injected into the control group.The brain water content was measured using wet-weight/dry-weight method one day after injection; enhanced Perl＇s reaction was used for iron staining one,three and 28 d after injection; brain HO-1 and ferritin were examined by Western blotting one day after and by immunohistochemistry one,three and 28 d after injection.Results One d after PIVH onset,edema in the ipsilateral hemisphere was increased as compared with control one （P〈0.05）.One,three and 28 days after PIVH,significant iron overload in the perihematomal tissues and a large number of iron staining positive cells in the surrounding tissues of ventricle wall were observed,while no iron overload was noted in the control group.Immunohistochemical staining showed a lot of HO-1 positive cells and ferritin positive cells in the right basal ganglia and periventricular areas of the experimental group,while the control group could note few.Western blotting indicated that PIVH could up-regulate HO-1 and ferritin expressions as compared with controls one day after injection （P〈0.05）.Conclusion The iron overload and upregulation of iron-handling proteins,including heme oxygenase-1 and ferritin,in the brain after PIVH suggest that iron could be a target for PIVH therapy.