中文摘要：

目的探讨绿茶多酚（GTPs）对牛内皮细胞窖蛋白-1（Cav-1）表达的影响。方法以体外原代培养的牛主动脉内皮细胞（BAECs）为研究对象;以4μg/ml的绿茶多酚分别作用于牛内皮细胞0,4,8,12,16和24 h,免疫印迹方法观察Cav-1的蛋白水平变化;RT-PCR检测窖蛋白-1的mRNA表达水平变化。结果Cav-1蛋白和mRNA表达水平在4μg/ml GTPs作用4 h开始下降,可以维持到8 h,在12 h后Cav-1蛋白和mRNA恢复到正常水平。结论绿茶多酚可降低内皮细胞窖蛋白-1的蛋白和mRNA表达,并且有时效性,可能是茶及其多酚改善内皮功能和预防心血管疾病的分子机制之一。

英文摘要：

Objective To investigate the influence of green tea polyphenols（GTPs） on caveolin-1 （Cav-1） eXpression in bovine endothe/ial cells in the level of cell and molecular initially. Methods In vitro, primary cultured bovine artery endothelial cells （BAECs） were treated with 4μg/ml GTPs for 0, 4, 8, 12, 16 and 24 hours, respectively. After protein and RNA extracted from treated cells, the Cav-1 protein and mRNA levels were detected by Western Blot and RT-PCR methods. Results After BAECs co-incubated with 4 μg/ml GTPs for 4 hours, the expression of Car-1 protein and mRNA decreased. The down-regulation effect of GTPs can maintain 4 hours and protein and mRNA expression of Cav-1 regained to normal level after 8 hours. Conclusion GTPs can decrease the protein and mRNA expression of Cav-1 in endothelial cells, and the influence of GTPs may be chronergy. It may be the molecular mechanism of tea amelioration endothelial function and prevention CVI）.